In Vitro Methods in Toxicology

The volume brings together the once disparate knowledge of cultural techniques and thus provides an important reference work for industrial and academic toxicologists.

C. K. Atterwill (Edited by), C. E. Steele (Edited by)

9780521104753, Cambridge University Press

Paperback / softback, published 19 March 2009

472 pages
22.9 x 15.2 x 2.7 cm, 0.69 kg

First published in 1987, this book describes tissue (cell and organ) culture techniques which could be used at a critical predevelopment stage to screen for drug-inducing toxicity or to analyse the molecular mechanisms of toxicity at a later stage in parallel with investigative studies. This type of research has important ethical, financial and scientific implications. In particular, less animals and compound are required in in vitro research and more detailed information at the cellular and subcellular levels can be obtained. The volume brings together the once disparate knowledge of cultural techniques and thus provides an important reference work for industrial and academic toxicologists. It could also form a framework for discussion of potential improvements and modification of toxicity testing.

Introduction G. B. Leslie and P. Johnson
Part I. Target Organ Toxicity: 1. In vitro methods in renal toxicology M. A. Snith, W. R. Hewitt and J. Hook
2. Ototoxicity E. S. Harpur
3. Cultured cell models for studying problems in cardiac toxicology N. Sperelakis and G. Bkaily
4. Use of the isolated rat heart for the detection of cardiotoxic agents A. Cockburn
5. Brain reaggregate cultures in neurotoxicological investigations C. K. Atterwill
6. Application of thyroid cell culture to the study of thyrotoxicity C. G. Brown
7. In vitro methods to investigate toxic lung disease A. Poole and R. C. Brown
8. Metabolism and toxicity of drugs in mammalian hepatocyte culture R. J. Chenery
9. In vitro evaluation of haemic systems in toxicology J. S. H. Luke and G. R. Betton
Part II. General and Topical Toxicity: 10. The chorioallantoic membrance in irritancy testing R. S. Lawrence
11. In vitro dermal irritancy tests P. A. Duffy and O. P. Flint
12. Genetic toxicology M. Richold
13. In vitro immunotoxicology K. Miller, S. Nicklin and S. G. Volsen
Part III. Reproductive Toxicity: 14. An in vitro test for teratogens using cultures of rat embryo cells O. P. Flint
15. Postimplantion embryo culture and its application to problems in teratology D. L. Cockroft and C. E. Steele
16. Sub-mammalian and sub-vertebrate models in teratogenicity screening S. J. Freeman and N. A. Brown
17. The use of in vitro techniques to investigate the action of testicular toxicants D. A. Garside
18. An in vitro assessment of ovarian function: a potential tool for investigating mechanisms of toxicity in the ovary D. A. Garside and P. McKibbin
Part IV. Conclusion: 19. Conclusion C. K. Atterwill and C. E. Steele
Index.

Subject Areas: Pharmacology [MMG]