In Situ Molecular Pathology and Co-expression Analyses

Continues to empower the next generation of researchers and diagnosticians to include in situ molecular pathology in their work by using easy-to-follow and easy-to-understand protocols for in situ hybridization, immunohistochemistry, and co-expression analyses

Gerard J. Nuovo (Author)

9780128206539, Elsevier Science

Paperback, published 3 September 2020

418 pages, 250 illustrations (240 in full color)
27.6 x 21.5 x 2.6 cm, 1.18 kg

Praise for the First Edition:"If you perform this type of testing in your laboratory, you must get this book. When you think all experimental approaches for in situ molecular testing methods have failed, have faith and refer to this book for the solution." --Doody.com, 2013

Major advancements in the field of in situ molecular pathology have occurred since publication of the first edition. In Situ Molecular Pathology and Co-expression Analyses, Second Edition, continues to teach both the molecular basis for the improvements and the actual protocols. This is the unique feature that separates it from the pack of other "cook-book" type approaches. The fields of in situ hybridization and immunohistochemistry have expanded rapidly where computer-based analyses systems have greatly expanded the power of these methods. Further, knowledge of the marked improvements in the reagents themselves since the first edition can make the difference of excellent versus misleading data. The automated platforms require that researchers and diagnostic biomedical investigators have a good understanding of the basics of in situ based tests, protocols, and biochemistry for troubleshooting in order to maximize the use of these platforms. This second edition focuses attention on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. Practicing molecular pathologists, diagnostic pathologists, laboratory directors, and toxicologists, as well as clinicians and researchers in training, will benefit from this clear presentation of protocols and theoretical framework. Data derived from in situ hybridization and immunohistochemistry.

1. Introduction2. The Basics of Molecular Pathology3. The Biochemical Basis of in situ Hybridization and Immunohistochemistry4. The Basics of in situ Hybridization5. The Basics of Immunohistochemistry6. Quality Control for Immunohistochemistry and in situ Hybridization: How to Know if the Color Change Is Signal or Background7. Recent Improvements in Immunohistochemistry and in situ Hybridization8. The Basics of Histologic Interpretations of Tissues9. The Recommended Protocol for in situ Hybridization10. The Recommended Protocol for Immunohistochemistry11. Coexpression Analyses12. PCR in situ Hybridization and RT in situ PCR

Subject Areas: Genetics [non-medical PSAK], Pathology [MMF], Physiology [MFG], Medical laboratory testing & techniques [MBGL]