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Imaging and Spectroscopic Analysis of Living Cells
Optical and Spectroscopic Techniques

This volume of Methods in Enzymology is the first of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells

P. Michael Conn (Volume editor)

9780123918574

Hardback, published 28 February 2012

560 pages
22.9 x 15.1 x 3.2 cm, 1.04 kg

Approx.512 pages

Part A: Techniques

  • Laser Induced Radiation Microbeam Technology and Simultaneous Real-time Fluorescence Imaging in Live Cells
  • A cell biologist’s guide to high resolution imaging
  • Applications of fluorescence lifetime spectroscopy and imaging to lipid domains in vivo
  • Detecting and Tracking Non-Fluorescent Nanoparticle Probes in Live Cells
  • Fluorescence lifetime microscopy of tumor cell invasion, drug delivery and cytotoxicity
  • Measuring membrane protein dynamics in neurons using Fluorescence recovery after photobleach (FRAP)
  • Distinctive characteristics of Madeira wine regarding its traditional winemaking and modern analytical methodologies
  • Green to red photo-convertible mEosFP aided live imaging in plants
  • Methods for Cell and Particle Tracking
  • Correlative light-electron microscopy: a potent tool for the imaging of rare or unique cellular and tissue events and structures
  • Optical Techniques for Imaging Membrane Domains in Live Cells (Live Cell Palm of Protein Clustering)
  • Single live cell topography and activity imaging with the Shear-Force-Based constant-distance scanning electrochemical microscope
  • Visualization of TGN-endosome trafficking in mammalian and drosophila cells
  • Live cell imaging with chemical specificity using dual frequency CARS microscopy
  • Imaging intracellular protein dynamics by spinning disk confocal microscopy

Part B: Tools

  • Visualizing Dynamic Activities of Signaling Enzymes using genetically encodable FRET-based biosensors: from designs to applications
  • Live-cell Imaging of Aquaporin-4 Supramolecular Assembly and Diffusion
  • Coiled-coil tag–probe labeling methods for live-cell imaging of membrane receptors
  • Monitoring Protein Interactions in Living Cells with Fluorescence Lifetime Imaging Microscopy
  • Open Source Tools for Fluorescent Imaging
  • Nanoparticle PEBBLE sensors in live cells

Subject Areas: Biochemistry [PSB], Medical imaging [MMP]

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