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Identification and Expression of G Protein-Coupled Receptors

Kevin R. Lynch (Edited by), KR Lynch (Author)

9780471194934, Wiley

Paperback / softback, published 26 May 1999

239 pages
25.2 x 19 x 1.2 cm, 0.425 kg

Ein Buch für Ihren Labortisch! G-Protein-Rezeptoren sind die primären Effektoren für unseren Geschmacks- und Gesichtssinn. Im Mittelpunkt dieser Monographie stehen neueste Ansätze zur Identifikation von Liganden der GCPRs, wobei der Schwerpunkt auf der Expression, nicht auf Klonierungsverfahren liegt. Die ausführlichen Versuchsanleitungen werden von den Autoren kommentiert, Vor- und Nachteile sind präzise angegeben. (05/98)

Cloning of G Protein-Coupled Receptor Genes: The Use of Homology Screening and the Polymerase Chain Reaction (A. Marchese, et al.).

Practical Approaches for Cloning G Protein-Coupled Receptors Using Mammalian Cell Expression (T. Murphy).

G Protein-Coupled Receptor Informatics and the Orphan Problem (K. Lynch).

The Use of Xenopus laevis Oocytes for the Study of G Protein-Coupled Receptors (A. Lee & M. Durieux).

Stable Expression of G Protein-Coupled Receptors in Mammalian Cells (K. Neve & R. Neve).

Heterologous Expression in Primary Cell Cultures (R. Leonard).

Expression of G Protein-Coupled Receptors in Escherichia coli (R. Grisshammer).

Large-Scale Expression/Purification of G Protein-Coupled Receptors Mammalian Cells (A. Robeva & J. Linden).

High-Level Expression of G Protein-Coupled Receptors in the Baculovirus/SF9 Cell Expression System (N. Rondeau, et al.).

Expression of G Protein-Coupled Receptors in Drosophila Schneider 2 Cells (M. Graziano, et al.).

Heterologous G Protein-Coupled Receptors Expressed in Saccharomyces cerevisiae: Methods for Genetic Analysis and Ligand Identification (M. Pausch, et al.).

Index.

Subject Areas: Biology, life sciences [PS]

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