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High-Throughput Screening Methods in Toxicity Testing
Pablo Steinberg (Edited by), P Steinberg (Author)
9781118065631, Wiley
Hardback, published 26 April 2013
570 pages
24.1 x 16.3 x 3.4 cm, 0.939 kg
Explores the benefits and limitations of the latest high-throughput screening methods With its expert coverage of high-throughput in vitro screening methods for toxicity testing, this book makes it possible for researchers to accelerate and streamline the evaluation and risk assessment of chemicals and drugs for toxicity. Moreover, it enables them to comply with the latest standards set forth by the U.S. National Research Council's "Toxicity Testing in the 21st Century: A Vision and Strategy" and the E.U.'s REACH legislation. Readers will discover a variety of state-of-the-science, high-throughput screening methods presented by a group of leading authorities in toxicology and toxicity testing. High-Throughput Screening Methods in Toxicity Testing is divided into five parts: Each chapter describes method principles and includes detailed information about data generation, data analysis, and applications in risk assessment. The authors not only enumerate the advantages of each high-throughput method over comparable conventional methods, but also point out the high-throughput method's limitations and potential pitfalls. In addition, the authors describe current research efforts to make high-throughput toxicity screening even more cost effective and streamlined. Throughout the book, readers will find plenty of figures and illustrations to help them understand and perform the latest high-throughput toxicity screening methods. This book is ideal for toxicologists and other researchers who need to implement high-throughput screening methods for toxicity testing in their laboratories as well as for researchers who need to evaluate the data generated by these methods.
PREFACE ix CONTRIBUTORS xi PART I GENERAL ASPECTS 1 ToxCast: Predicting Toxicity Potential Through High-Throughput Bioactivity Profiling 3 2 High-Throughput Toxicity Testing in Drug Development: Aims, Strategies, and Novel Trends 33 3 Incorporating Human Dosimetry and Exposure Information with High-Throughput Screening Data in Chemical Toxicity Assessment 77 4 The Use of Human Embryonic Stem Cells in High-Throughput Toxicity Assays 97 PART II HIGH-THROUGHPUT ASSAYS TO ASSESS DIFFERENT CYTOTOXICITY ENDPOINTS 6 High-Throughput Flow Cytometry Analysis of Apoptosis 129 7 High Content Imaging-Based Screening for Cellular Toxicity Pathways 143 8 The Keratinosens Assay: A High-Throughput Screening Assay to Assess Chemical Skin Sensitization 159 9 High-Throughput Screening Assays to Assess Chemical Phototoxicity 177 PART III HIGH-THROUGHPUT ASSAYS TO ASSESS DNA DAMAGE AND CARCINOGENESIS 10 Ames IITM and Ames Liquid Format Mutagenicity Screening Assays 193 11 High-Throughput Bacterial Mutagenicity Testing: VitotoxTM Assay 213 12 Genotoxicity and Carcinogenicity: Regulatory and Novel Test Methods 233 13 High-Throughput Genotoxicity Testing: The Greenscreen Assay 271 14 High-Throughput Assays to Quantify the Formation of DNA Strand Breaks 285 15 High-Throughput Versions of the Comet Assay 295 16 Automated Soft Agar Colony Formation Assay for the High-Throughput Screening of Malignant Cell Transformation 309 17 High-Throughput Quantification of Morphologically Transformed Foci in Bhas 42 Cells (v-Ha-ras Transfected BALB/c 3T3) Using Spectrophotometry 317 PART IV HIGH-THROUGHPUT ASSAYS TO ASSESS REPRODUCTIVE TOXICITY, CARDIOTOXICITY, 18 ReProGlo: A New Stem-Cell-Based High-Throughput Assay to Predict the Embryotoxic Potential of Chemicals 343 19 Embryonic Stem Cell Test (EST): Molecular Endpoints Toward High-Throughput Analysis of Chemical Embryotoxic Potential 357 20 Zebrafish Development: High-Throughput Test Systems to Assess Developmental Toxicity 371 21 Single Cell Imaging Cytometry-Based High-Throughput Analysis of Drug-Induced Cardiotoxicity 385 22 High-Throughput Screening Assays to Evaluate the Cardiotoxic Potential of Drugs 403 23 High-Throughput Screening Assays to Evaluate the Hematotoxic Potential of Drugs 421 PART V HIGH-THROUGHPUT ASSAYS TO ASSESS DRUG METABOLISM AND RECEPTOR-RELATED TOXICITY 24 High-Throughput Enzyme Biocolloid Systems for Drug Metabolism and Genotoxicity Profiling Using LC–MS/MS 433 25 Higher-Throughput Screening Methods to Identify Cytochrome P450 Inhibitors and Inducers: Current Applications and Practice 453 26 High-Throughput Yeast-Based Assays to Study Receptor-Mediated Toxicity 479 27 Evaluating the Peroxisomal Phenotype in High Content Toxicity Profiling 501 28 A Panel of Quantitative Calux R Reporter Gene Assays for Reliable High-Throughput Toxicity Screening of Chemicals and Complex Mixtures 519 29 DR-Calux R : A High-Throughput Screening Assay for the Detection of Dioxin and Dioxin-Like Compounds in Food and Feed 533 INDEX 547
Keith A. Houck, Ann M. Richard, Richard S. Judson, Matthew T. Martin, David M. Reif, and Imran Shah
Willem G.E.J. Schoonen, Walter M.A. Westerink, Femke M. van de Water, and Horbach G. Jean
Barbara A. Wetmore and Russell S. Thomas
Xin Huang, Dan-yan Zhu, and Yi-jia Lou
5 High-Throughput Screening Assays for the Assessment of Cytotoxicity 109
Andrew L. Niles, Richard A. Moravec, Tracy J. Worzella, Nathan J. Evans, and Terry L. Riss
Francesca de Giorgi and Franc¸ois Ichas
Bram Herpers and Bob van de Water
Andreas Natsch
Satomi Onoue, Yoshiki Seto, and Shizuo Yamada
Kamala Pant
Luc Verschaeve
Walter M.A. Westerink, Joe C.R. Stevenson, G. Jean Horbach, Femke M. van de Water, Beppy van de Waart, and Willem G.E.J. Schoonen
Jorg Blumel and Nadine Krause
Marýa Moreno-Villanueva and Alexander Burkle
Irene Witte and Andre Stang
Pablo Steinberg
Kiyoshi Sasaki, Ayako Sakai, and Noriho Tanaka
AND HAEMATOTOXICITY
Frederik Uibel and Michael Schwarz
Peter T. Theunissen, Esther de Jong, Joshua F. Robinson, and Aldert H. Piersma
Stephanie Padilla
Min Jung Kim and Joon Myong Song
Carl-Fredrik Mandenius and Thomas Meyer
Caroline Haglund, Rolf Larsson, and Martin Hoglund
James F. Rusling and John Schenkman
David M. Stresser and George Zhang
Johanna Rajasarkka and Marko Virta
Jonathan Z. Sexton and Kevin P. Williams
Bart van der Burg, Sander van der Linden, Hai-yen Man, Roos Winter, Lydia Jonker, Barbara van Vugt-Lussenburg, and Abraham Brouwer
Barbara van Vugt-Lussenburg, Harrie T. Besselink, Bart van der Burg, and Abraham Brouwer
Subject Areas: Chemistry [PN]
