Handbook of Isoelectric Focusing and Proteomics

A comprehensive handbook for those working in this mature and exciting field

David Garfin (Edited by), Satinder Ahuja (Edited by)

9780120887521

Hardback, published 29 September 2005

380 pages
24 x 16.5 x 2.6 cm, 0.84 kg

Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics.

Preface.

Incidents of Travel in IEF and IPGs.

Overview.

Evolution and Development of IEP.

Theory and Simulation of IEF.

Generation of pH Gradients.

Slab Gel IEF.

Two-Dimensional Gel Electrophoresis.

Some Practices and Pitfalls of Sample Preparation for IEF in Proeomics.

Protein Detection and Imaging in IEF Gels.

Capillary IEF.

Free Flow IEF.

Isoelectric Focusing and Proteomics.

Chromatofocusing.

Alternative Forms of IEF.

Subject Areas: Electronics & communications engineering [TJ], DNA & Genome [PSAK1], Analytical chemistry [PNF]