General Techniques of Cell Culture

Concise introduction to a major technique of cell biology laboratories for those new to the field.

Maureen A. Harrison (Author), Ian F. Rae (Author)

9780521574969, Cambridge University Press

Paperback, published 13 October 1997

176 pages, 17 b/w illus. 10 tables
22.9 x 15.2 x 1 cm, 0.27 kg

'The best thing about this book is its conciseness and its introductory chapters … and sensible advice … Tissue culture is not easy to describe and they make a pretty good job of it.' Janet Smith, BDSB Newsletter

Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a tissue culture facility, including tissue culture media and sera. It describes methods for growing suspension and adhesion cultures, including how to store cells and prepare primary cultures from cells. For those already culturing cells, the handbook will act as a handy reference to the basic techniques. The essence of the book is to deal with the generalities of cell culture to give a grasp of the basic concepts before involvement in more specialized work in the field. Ideal for anyone moving into tissue cell culture techniques or looking for a concise reference book.

Preface
1. Introduction
2. Equipment
3. Media
4. Sera
5. Cell culture
6. Preparation of primary cells
7. Cryopreservation
8. Quality control
9. Specialized techniques
10. Health and safety
Acknowledgements
Glossary, Index.

Subject Areas: Cellular biology [cytology PSF], Scientific equipment, experiments & techniques [PDN]