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Gene therapy
Potential Applications of Nanotechnology
Surendra Nimesh (Author)
9781907568404, Elsevier Science
Hardback, published 31 October 2013
380 pages
23.3 x 15.6 x 2.6 cm, 0.7 kg
Gene therapy is emerging as a new class of therapeutics for the treatment of inherited and acquired diseases. However, poor cellular uptake and instability of DNA in the physiological milieu limits its therapeutic potential, hence a vector which can protect and efficiently transport DNA to the target cells must be developed. Nanotechnology-based non-viral vectors have been proposed as potential candidates. Various polymeric nanoparticles have been shown to be suitable, with high cellular uptake efficiencies and reduced cytotoxicity. These delivery vectors form condensed complexes with DNA which result in shielding against enzymatic degradation and enhanced cellular targeting. Advantages including easy manipulatibility, high stability, low cost and high payload, mean that nanoparticles from various polymers have been exploited. Gene therapy gives a systematic account of the many aspects of nanotechnology mediated gene therapy, from the preparation of nanoparticles to physicochemical characterization, and follows with applications in in vitro and in vivo models. This book emphasizes the various aspects of nanotechnology-based gene therapy, with initial chapters detailing the tools and techniques available for preparation and in vitro and in vivo characterization of nanoparticles. Later chapters provide exhaustive details on polymeric systems employed for gene therapy.
Dedication List of figures and tables Acknowledgments Foreword Preface About the author Chapter 1: Nanotechnology: an introduction Abstract: 1.1 Introduction 1.2 Definition of nanotechnology 1.3 Structure of the book Chapter 2: Methods of nanoparticle preparation Abstract: 2.1 Introduction 2.2 Preparation of nanoparticles by polymerization of monomers 2.3 Preparation of nanoparticles using preformed polymers 2.4 Methods of controlled release Chapter 3: Tools and techniques for physico-chemical characterization of nanoparticles Abstract: 3.1 Introduction 3.2 Physico-chemical characterization Chapter 4: Characterization of nanoparticles: in vitro and in vivo Abstract: 4.1 Introduction 4.2 In vitro characterization of nanoparticles 4.3 In vivo characterization 4.4 Conclusions Chapter 5: Theory and limitations to gene therapy Abstract: 5.1 Introduction 5.2 Mechanism of gene delivery 5.3 Barriers to gene delivery 5.4 Conclusions Chapter 6: Targeted gene delivery mediated by nanoparticles Abstract: 6.1 Introduction 6.2 Approaches for targeted gene delivery 6.3 Conclusions Chapter 7: Polymeric nanoparticles for gene delivery Abstract: 7.1 Introduction 7.2 Advantages of nanoparticles 7.3 Limitations of nanoparticles 7.4 Conclusions Chapter 8: Poly-L-lysine nanoparticles Abstract: 8.1 Introduction 8.2 In vitro and in vivo applications of poly-L-lysine/DNA nanoparticles 8.3 Polylysine-containing peptides for gene delivery 8.4 Conclusions Chapter 9: Chitosan nanoparticles Abstract: 9.1 Introduction 9.2 Factors affecting transfection efficiency of chitosan nanoparticles 9.3 Conclusions Chapter 10: Polyethylenimine nanoparticles Abstract: 10.1 Introduction 10.2 Derivatives of PEI for in vitro and in vivo gene delivery 10.3 Degradable PEI for gene delivery 10.4 Conclusions Chapter 11: Atelocollagen Abstract: 11.1 Introduction 11.2 Atelocollagen-mediated gene delivery 11.3 Conclusions Chapter 12: Protamine nanoparticles Abstract: 12.1 Introduction 12.2 Protamine nanoparticles for gene delivery 12.3 Liposome/protamine/ DNA complexes 12.4 Protamine conjugation to other ligands 12.5 Conclusions Chapter 13: Dendrimers Abstract: 13.1 Introduction 13.2 Dendrimers in gene delivery 13.3 Conclusions Chapter 14: Cyclodextrins and cyclodextrin-containing polymers Abstract: 14.1 Introduction 14.2 Cyclodextrin-embedded polymers 14.3 Polymers with cyclodextrins as pendant groups 14.4 Cyclodextrins as adjuvants for enhanced gene delivery 14.5 Cyclodextrin-based polyrotaxanes 14.6 Conclusions Chapter 15: Poly(D,L-lactide-co-glycolide)-based nanoparticles Abstract: 15.1 Introduction 15.2 PLGA nanoparticles for gene delivery 15.3 Chitosan-modified PLGA nanoparticles 15.4 Polyethylenimine-modified PLGA nanoparticles 15.5 Other modifications to PLGA nanoparticles 15.6 Conclusions Chapter 16: Metallic and inorganic nanoparticles Abstract: 16.1 Introduction 16.2 Gold nanoparticles 16.3 Mesoporous silica nanoparticles 16.4 MSN for gene delivery 16.5 Polycation-modified MSN for gene delivery 16.6 Conclusions Index
Subject Areas: Molecular biology [PSD], DNA & Genome [PSAK1]
