G Protein Coupled Receptors
Structure

This new volume of Methods in Enzymology continues the legacy of this premier with quality chapters authored by leaders in the field. This volume covers G protein coupled receptors including such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.

P. Michael Conn (Volume editor)

9780123918611, Elsevier Science

Hardback, published 15 March 2013

440 pages
22.9 x 15.1 x 2.8 cm, 0.72 kg

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.

1. G Protein Coupled Receptors: Techniques for Probing Structure
2. Comformational Ensemble View of G Protein-Coupled Receptors and The Effect of Mutations and Ligand Binding
3. Structural Evolution of G-Protein-Coupled Receptors: A Sequence Space Approach
4. Directed Evolution of G Protein-Coupled Receptors for High Functional Expression and Detergent Stability
5. The Role of Hydrophobic Amino Acids in the Structure and Function of the Rhodopsin Family of G Protein-Coupled Receptors
6. Structure of ß-Adrenergic Receptors
7. Advances in Methods to Characterize Ligand-Induced Ionic Lock and Rotamer Toggle Molecular Switch in G-Protein Coupled Receptors
8. Crystallogenesis of Adenosine A_2A Receptor – T4 Lysozyme Fusion Protein: A Practical Route for the Structure
9. Probing GPCR Structure: Adenosine and P2Y Nucleotide Receptors
10. Strategies for Studying the Ligand Binding Site of GPCRs: Photoaffinity Labeling of the VPAC1 Receptor, a Prototype of Class B GPCRs
11. Expression of Mammalian G Protein-Coupled Receptors in Caenorhabditis Elegans
12. Expression, Purification and Structural Analysis of Intracellular C-Termini from Metabotropic Glutamate Receptors
13. Unnatural Amino Acid Mutagenesis of GPCRs Using Amber Codon Suppression and Bioorthogonal Labeling
14. Mapping a Ligand Binding Site Using Genetically-Encoded Photoactivatable Crosslinkers
15. Alternative mRNA Splicing of G Protein-Coulped Receptors
16. Functional Residues of the CB1 Cannabinoid Receptor

 

Subject Areas: Molecular biology [PSD], Biochemistry [PSB], Cellular physiology [MFGC]