Fluorescence Fluctuation Spectroscopy (FFS), Part A

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis.

Sergey Tetin (Volume editor)

9780123884220

Hardback, published 21 February 2013

336 pages
22.9 x 15.1 x 2.4 cm, 0.63 kg

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation SpectroscopyContains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.

1. 40 Years of FCS: How it all Began
2. Brief Introduction to Fluorescence Correlation Spectroscopy
3. Dual-Color Fluorescence Cross-Correlation Spectroscopy (FCCS) with continuous Laser Excitation in a Confocal Setup
4. Brightness Analysis
5. Time-Integrated Fluorescence Cumulant Analysis and its Application in Living Cells
6. Raster Image Correlation Spectroscopy and Number and Brightness Analysis
7. Global Analysis in Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Microscopy

8. Dual-focus Fluorescence Correlation Spectroscopy (2fFCS)
9. Pulsed Interleaved Excitation: Principles and Applications
10. Image Correlation Spectroscopy: Mapping Correlations in Space, Time and Reciprocal Space

Subject Areas: Enzymology [PSBZ], Spectrum analysis, spectrochemistry, mass spectrometry [PNFS]