Experiments in Plant Tissue Culture

This new edition of a highly successful book has been completely revised and updated, and features new illustrations and experiments.

John H. Dodds (Author), Lorin W. Roberts (Author), J. Heslop-Harrison (Foreword by)

9780521478922, Cambridge University Press

Paperback, published 27 January 1995

276 pages, 25 b/w illus. 7 tables
22.8 x 15.2 x 1.4 cm, 0.36 kg

'… a worthy text and an excellent addition to any plant science laboratory.' John Love, British Society for Developmental Biology Newsletter

In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures, amongst others. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations added, together with new experiments on such topics as potato callus formation, and embryo culture.

Part I. Background Information: 1. Culture of plant cells, tissues, and organs
2. Laboratory facilities
3. Aseptic techniques
4. Media composition and preparation
Part II. Experimental: Callus and Callus-Derived Systems: 5. Initiation and maintenance of callus
6. Organogenesis
7. Cell suspensions
8. Somatic embryogenesis
Part III. Experimental: Culture of Organs and Organized Systems: 9. Isolated roots
10. Micropropagation by bud proliferation
11. Anther and pollen cultures
Part IV. Experimental: Isolated Cells: 12. Transdifferentiation of parenchyma cells to tracheary elements
13. Isolation and culture of protoplasts
14. Protoplast fusion and somatic hybridization
Part V. Supplementary Topics: 15. Cryopreservation of germplasm
16. Production of secondary metabolites
17. Quantitation of procedures
18. Formulations of tissue culture media
19. Commercial sources of supplies
Author index
Subject index.

Subject Areas: Botany & plant sciences [PST], Cellular biology [cytology PSF]