Experimental Design for Laboratory Biologists
Maximising Information and Improving Reproducibility

A guide to designing lab-based biological experiments that have low bias, high precision and widely applicable results.

Stanley E. Lazic (Author)

9781107424883, Cambridge University Press

Paperback / softback, published 8 December 2016

422 pages, 124 b/w illus.
24.5 x 19 x 2 cm, 0.94 kg

'There is an increasing need to better design experiments not only to reduce the number of any animals being used in any such work, but also to ensure that the data so produced is meaningful. As part of that process knowing how to power studies and then properly analyse the data so generated is vital, and of late there have been concerns that this is not been done to same vigour as that seen in the clinical arena. However, most scientists struggle with this aspect of their work, and thus it is really refreshing to come across a book that explicitly deals with experimental design and analysis. This new book clearly lays out what can and should be done and is written by an acknowledged expert and I have no doubt that this book will become a recommended read for all those contemplating undertaking work of this type.' Roger Barker, University of Cambridge

Specifically intended for lab-based biomedical researchers, this practical guide shows how to design experiments that are reproducible, with low bias, high precision, and widely applicable results. With specific examples from research using both cell cultures and model organisms, it explores key ideas in experimental design, assesses common designs, and shows how to plan a successful experiment. It demonstrates how to control biological and technical factors that can introduce bias or add noise, and covers rarely discussed topics such as graphical data exploration, choosing outcome variables, data quality control checks, and data pre-processing. It also shows how to use R for analysis, and is designed for those with no prior experience. An accompanying website (https://stanlazic.github.io/EDLB.html) includes all R code, data sets, and the labstats R package. This is an ideal guide for anyone conducting lab-based biological research, from students to principle investigators working in either academia or industry.

1. Introduction: 1.1 What is reproducibility?
1.2 The psychology of scientific discovery
1.3 Are most published results wrong?
1.4 Frequentist statistical interference
1.5 Which statistics software to use?
2. Key ideas in experimental design: 2.1 Learning versus confirming experiments
2.2 The fundamental experimental design equation
2.3 Randomisation
2.4 Blocking
2.5 Blinding
2.6 Effect type: fixed versus random
2.7 Factor arrangement: crossed versus nested
2.8 Interactions between variables
2.9 Sampling
2.10 Use of controls
2.11 Front-aligned versus end-aligned designs
2.12 Heterogeneity and confounding
3. Replication (what is 'N'?): 3.1 Biological units
3.2 Experimental units
3.3 Observational units
3.4 Relationship between units
3.5 How is the experimental unit defined in other disciplines?
4. Analysis of common designs: 4.1 Preliminary concepts
4.2 Background to the designs
4.3 Completely randomised designs
4.4 Randomised block designs
4.5 Split-unit designs
4.6 Repeated measures designs
5. Planning for success: 5.1 Choosing a good outcome variable
5.2 Power analysis and sample size calculations
5.3 Optimal experimental designs (rules of thumb)
5.4 When to stop collecting data?
5.5 Putting it all together
5.6 How to get lucky
5.7 The statistical analysis plan
6. Exploratory data analysis: 6.1 Quality control checks
6.2 Preprocessing
6.3 Understanding the structure of the data
Appendix A. Introduction to R
Appendix B. Glossary.

Subject Areas: Popular medicine & health [VFD], Family & health [VF], Health & personal development [V], Biology, life sciences [PS], Mathematical modelling [PBWH], Applied mathematics [PBW], Mathematics [PB], Mathematics & science [P], Medicine [M]