Expansion Microscopy for Cell Biology

Compiles protocols and reviews on expansion microscopy techniques for Cell Biology, guiding researchers to choose  appropriate methods

Paul Guichard (Volume editor), Virginie Hamel (Volume editor)

9780128208076, Elsevier Science

Hardback, published 18 January 2021

356 pages
23.4 x 19 x 2.5 cm, 0.95 kg

Expansion Microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more.

1. Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion Paul Tilberg 2. Ultrastructure Expansion Microscopy (U-ExM) Davide Gambarotto, Virginie Hamel and Paul Guichard 3. Expansion STED microscopy (ExSTED) Helge Ewers 4. Simple multi-color super-resolution by X10 microscopy Sven Truckenbrodt 5. Expansion microscopy imaging of various neuronal structures Jae-Byum Chang 6. Mapping the neuronal cytoskeleton using expansion microscopy Lukas Kapitein 7. Mechanical expansion microscopy Bo Wang 8. Enhanced expansion microscopy to measure nanoscale structural and biochemical remodelling in single cells Izzy Jayasinghe 9. Application of Expansion Microscopy on Developing Arabidopsis Seeds Michael D. Nodine 10. A protocol to expand plant nuclei Veit Schubert 11. Expansion microscopy of the mitotic spindle Ivana Ponjavic 12. Expansion microscopy at the nanoscale. The nuclear pore complex as fiducial landmark Paolo Bianchini 13. Post-labeling expansion microscopy, a promise to go beyond super-resolution limitations Paul Guichard and Virginie Hamel 14. Ex-dSTORM and automated quantitative image analysis of expanded filamentous structures Markus Sauer 15. Expansion Microscopy on Drosophila Spermatocyte Centrioles Alan Wainman

Subject Areas: Cellular biology [cytology PSF], Molecular biology [PSD], Developmental biology [PSC]