Enzymology at the Membrane Interface: Intramembrane Proteases

Covers a subset of enzymes that work in the environment of the biological cell membrane, including isolation approaches and study

Michael H Gelb (Volume editor)

9780128122136

Hardback, published 5 January 2017

494 pages
22.9 x 15.1 x 3 cm, 0.93 kg

Enzymology at the Membrane Interface: Intramembrane Proteases, Volume 584, the latest release in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.

1. Biochemical Characterization of Function and Structure of RseP, an Escherichia Coli S2P Protease Y. Hizukuri, K. Akiyama and Y. Akiyama 2. Signal Peptidase Enzymology and Substrate Specificity Profiling R.E. Dalbey, D. Pei and Ö.D. Ekici 3. Screening and Characterization Strategies for Nanobodies Targeting Membrane Proteins S. Veugelen, M. Dewilde, B.D. Strooper and L. Chávez-Gutiérrez 4. Probing the Activity of Eukaryotic Rhomboid Proteases in Vitro B. Cordier and M.K. Lemberg 5. Expression, Purification, and Enzymatic Characterization of Intramembrane Proteases R. Zhou, Y. Shi and G. Yang 6. Analyzing Amyloid-ß Peptide Modulation Profiles and Binding Sites of ?-Secretase Modulators J. Trambauer, A. Fukumori, B. Kretner and H. Steiner 7. Probing the Structure and Function Relationships of Presenilin by Substituted-Cysteine Accessibility Method T. Tomita 8. A New Method to Determine the Transmembrane Conformation of Substrates in Intramembrane Proteolysis by Deep-UV Resonance Raman Spectroscopy J.W. Cooley, A. Abdine, M. Brown, J. Chavez, B. Lada, R.D. JiJi and I. Ubarretxena-Belandia 9. An Inducible Reconstitution System for the Real-Time Kinetic Analysis of Protease Activity and Inhibition Inside the Membrane R.P. Baker and S. Urban 10. Production of Recombinant Rhomboid Proteases E. Arutyunova, R. Panigrahi, K. Strisovsky and M.J. Lemieux 11. Mechanism and Inhibition of Rhomboid Proteases K. Strisovsky 12. Enzymatic Assays for Studying Intramembrane Proteolysis D.M. Bolduc, D.J. Selkoe and M.S. Wolfe 13. Methods for S¬tructural and Functional Analyses of Intramembrane Prenyltransferases in the UbiA Super Family Y. Yang, N. Ke, S. Liu and W. Li 14. Functional Study of the Vitamin K Cycle Enzymes in Live Cells J.-K. Tie and D.W. Stafford 15. Activity Assays for Rhomboid Proteases E. Arutyunova, K. Strisovsky and J. Lemieux

Subject Areas: Cellular biology [cytology PSF], Molecular biology [PSD], Enzymology [PSBZ]