Enzyme Kinetics
From Diastase to Multi-enzyme Systems

Arthur R. Schulz (Author)

9780521445009, Cambridge University Press

Hardback, published 26 January 1995

260 pages
22.9 x 15.2 x 1.9 cm, 0.55 kg

"Currently, very few introductions to enzyme kinetics are available; this book could serve either as a text for a course in enqyme kinetics or as a reference for those needing an introduction to steady state enzyme kinetics. Definitely recommended." L.M. Prescott, Choice

This text covers the field of steady-state kinetics from basic principles to the control of the multi-enzyme systems which constitute metabolic pathways. Emphasis is placed on the interpretation of the kinetic behaviour of enzyme-catalyzed reactions in terms of mechanisms. Algorithms are developed which can be implemented in computer programs for the derivation of equations. The treatment of steady-state enzyme kinetics is extended to allosteric enzymes and subunit interactions in polymeric enzymes. Principles are presented which provide for mathematical analysis of the control of multi-enzyme systems. Problems are included at the end of each chapter and their solutions are found at the end of the book. This book will be a useful text for advanced undergraduates and graduate students taking courses in enzyme chemistry and enzyme kinetics.

Preface
Part I. Basic Steady State Enzyme Kinetics: 1. Derivation of a rate equation
2. A closer look at the basic assumptions
3. Enzyme inhibition
4. Reversible enzyme-catalyzed reactions
Part II. Enzyme Reaction Sequence: 5. Multi-reactant enzymic reactions
6. Analysis of multi-reactant enzyme kinetics
7. Prediction of reaction sequence
8. Enzyme-catalyzed isotopic exchange
9. Kinetic isotope effect on steady state parameters
10. The effect of pH on enzyme kinetics
Part III. Non-Hyperbolic Enzyme Kinetics: 11. The causes of non-hyperbolic enzyme kinetics
12. Analysis of non-hyperbolic enzyme kinetics
13. The effect of subunit interactions on enzyme kinetics
Part IV. Control of Multi-Enzyme Systems: 14. Control of linear multi-enzyme systems
15. Control of branched multi-enzyme systems
16. Biochemical systems theory
Part V. Solutions To Problems: Author index
Subject index.

Subject Areas: Molecular biology [PSD], Enzymology [PSBZ]