Endocrine Cell Culture

This book offers clear, affordable lab protocols and descriptive accounts of all major endocrine cell cultures.

Stephen Bidey (Edited by)

9780521595636, Cambridge University Press

Paperback, published 28 August 1998

168 pages, 45 b/w illus. 4 tables
22.9 x 15.2 x 0.9 cm, 0.23 kg

'Crystal clear cell culture advice. You'll want to wrap it in plastic and keep it on the lab bench.' Gareth Cuttle, BSDB Newsletter

This handy reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally-intact cells from mammalian endocrine tissues, and their maintenance as primary cultures. The cell types selected for coverage illustrate the diversity of endocrine tissues from which cells have been isolated, and the range of procedures which have been devised to ensure the optimal survival and behaviour of each cell type under study. Particular emphasis has been placed on the provision of detailed protocols describing, step by step, the manipulations necessary to establish differentiated and responsive cultures. The chapters have been prepared by authors having direct practical experience of the cell type concerned, and the reader is therefore provided with first-hand accounts on the background to each procedure, the avoidance of potential problems and pitfalls, and the fundamental questions in endocrinology which may be addressed using each cell culture model.

List of contributors
Preface
Acknowledgements
Introduction Stephen Bidey
1. Parathyroid cells Ronal R. Macgregor
2. Ovarian granulosa Denis A. Magoffin
3. Anterior pituitary cells Seon H. Shin and John V. Milligan
4. Pancreatic ß-cells Leonard Best
5. Adrenocortical and adrenomedullary cells Matthias M. Weber, Christian Fottner and Dieter Engelhardt
6. Leydig cells Frank Chuzel, Hervé Lejeune and José Saez
7. Thyroid follicular cells Margaret C. Eggo
8. Hypothalamic cells Hilary E. Murray, Duncan McKenzie and Glenda E. Gillies
Index.

Subject Areas: Cellular biology [cytology PSF], Scientific equipment, experiments & techniques [PDN]