Dynamics of Engineered Artificial Membranes and Biosensors

A state-of-the-art guide to building synthetic membranes for biological devices, covering their construction, measurement, and modelling.

William Hoiles (Author), Vikram Krishnamurthy (Author), Bruce Cornell (Author)

9781108423502, Cambridge University Press

Hardback, published 3 May 2018

470 pages, 161 b/w illus. 16 tables
25.3 x 17.8 x 2.6 cm, 1.09 kg

Learn about the state of the art in building artificial membranes and synthetic biological devices, and in constructing mathematical models for their dynamics at multiple time and spatial scales with this comprehensive book. Drawing on recent advances in bioengineering and biochemistry, it describes how to engineer tethered bilayer lipid membranes, bioelectronic interfaces, high-resolution biosensors, and diagnostic devices for non-invasive cellular measurements and electroporation. Multi-physics models combining atomistic (molecular dynamics and coarse-grained molecular dynamics), mesoscopic (Poisson–Nernst–Planck), and macroscopic (reaction-rate theory) dynamics provide a complete structure-to-function description of these devices. Experiments and dynamic models explain how anti-microbial peptides penetrate membranes, how molecular machine biosensors built out of artificial membranes can detect femtomolar concentrations, and how electroporation can be controlled. Supported by atomistic simulation code online, this is essential reading for researchers, students and professionals in bioengineering, chemical engineering, biophysics, applied mathematics, and electrical engineering.

Part I. Background: 1. Motivation and outline
2. Biochemistry for engineers: a short primer
3. Engineered artificial membranes
Part II. Building Engineered Membranes, Devices and Experimental Results: 4. Formation of engineered tethered membranes
5. Ion-channel switch biosensor
6. Physiochemical membrane platforms
7. Experimental measurement methods for engineered membranes
Part III. Dynamic Models for Artificial Membranes: Atoms-to-Device: 8. Reaction-rate constrained models for engineered membranes
9. Reaction-rate constrained models for the ICS biosensor
10. Diffusion constrained continuum models of engineered membranes
11. Electroporation models in engineered artificial membranes
12. Electroporation measurements in engineered membranes
13. Electrophysiological response of ion channels and cells
14. Coarse-grained molecular dynamics
15. All-atom molecular dynamics simulation models
16. Closing summary for part III: from atoms to device
Appendices: Appendix A. Elementary primer on partial differential equations (PDE)
Appendix B. Tutorial on coarse-grained molecular dynamics with peptides.

Subject Areas: Electronics engineering [TJF], Biotechnology [TCB], Nanotechnology [TBN], Biophysics [PHVN], Biomedical engineering [MQW], Biotechnology industries [KNDH1]