Drosophila Cells in Culture

New edition of the standard in the field of drosophila cell culture covers experimental procedures and protocols

Guy Echalier (Author), Norbert Perrimon (Author), Stephanie E Mohr (Author)

9780128094730

Paperback, published 30 November 2017

258 pages
22.9 x 15.1 x 1.7 cm, 0.43 kg

Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge.

1. Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophila Cells2. Primary Cell Cultures of Drosophila Embryonic Cells3. Continuous Drosophila Cell Lines Established In Vitro4. Cells or Tissues In Course of Differentiation5. Hormonal Control of The Development of Insects: Implications In Organ or Cell Cultures of Drosophila6. Gene Transfer into Cultured and Applications7. Rnai and Crispr (Cas9, Cas9-Act, Cas9-Repressor) Methods to Perturb Gene Function8. Functional Genomics Screens Using Rnai, Crispr, and Orf Overexpression

Subject Areas: Cellular biology [cytology PSF], Molecular biology [PSD], Developmental biology [PSC]