Diagnostic Molecular Biology

Describes the fundamentals of molecular biology in a clear, concise manner that includes current applications of clinical laboratory techniques

Chang-Hui Shen (Author)

9780323917889, Elsevier Science

Paperback, published 29 June 2023

588 pages
27.6 x 21.6 x 3.3 cm, 0.45 kg

Diagnostic Molecular Biology, Second Edition describes the fundamentals of molecular biology in a clear, concise manner with each technique explained within its conceptual framework and current applications of clinical laboratory techniques comprehensively covered. This targeted approach covers the principles of molecular biology, including basic knowledge of nucleic acids, proteins and chromosomes; the basic techniques and instrumentations commonly used in the field of molecular biology, including detailed procedures and explanations; and the applications of the principles and techniques currently employed in the clinical laboratory. Topics such as whole exome sequencing, whole genome sequencing, RNA-seq, and ChIP-seq round out the discussion.

1. Nucleic Acids: DNA and RNA 2. Nucleic Acid-Based Cellular Activities - DNA Replication, Damage, and Repair 3. Gene Expression: Transcription of the Genetic Code 4. Gene Expression: Translation of the Genetic Code 5. The Genome 6. Extraction and Purification of Nucleic Acids 7. Quantification and Analysis of Nucleic Acids 8. Extraction and Purification of Proteins 9. Quantification and Analysis of Proteins 10. Amplification of Nucleic Acids 11. Characterization of Nucleic Acids and Proteins 12. Techniques in Sequencing 13. Genome and Transriptome Analysis 14. Molecular Diagnosis of Chromosomal Disorders 15. Molecular Diagnosis of Gene Mutation and Inherited Diseases 16. Molecular Diagnosis of Infectious Diseases 17. The Biology of SARS-CoV-2 and the Molecular Diagnosis of COVID-19 18. Guidance for Molecular Diagnostic Laboratory

Subject Areas: Microbiology [non-medical PSG], Molecular biology [PSD]