Culture of Hematopoietic Cells

R. Ian Freshney (Edited by), RI Freshney (Author), Ian B. Pragnell (Edited by), Mary G. Freshney (Edited by)

9780471588306, Wiley

Paperback / softback, published 25 August 1994

296 pages
25.4 x 17.9 x 1.5 cm, 0.737 kg

Provides scientists with step-by-step methods for easy use in the lab when cultivating and experimenting with blood cells. Covering a range of human and mouse cell types, this laboratory manual includes contributions from more than twenty leading investigators who offer their practical expertise on a wide range of topics. Features detailed instructions for reagent preparation and information on the latest specialized equipment and supplies.

Partial table of contents:

PRIMITIVE PROGENITOR ASSAYS.

Cobblestone Area Forming Cell (CAFC) Assay (R. Ploemacher).

Assay for Murine Blast Cell Colonies (N. Katayama & M.Ogawa).

Serum-Deprived Cultures of Primary Hematopoietic Cells (G.Migliaccio & A. Migliaccio).

COLONY-FORMING ASSAYS FOR MALIGNANT CELLS.

Colony-Forming Assays for Acute Myeloid Leukemia (S. Rogers, etal.).

LONG-TERM ASSAYS AND IMMORTALIZATION.

Long-Term Culture of Human Myeloid Cells (H. Sutherland & C.Eaves).

Immortalization of Hematopoietic Cells (C. MacDonald).

PURIFICATION OF PROGENITOR CELLS.

Purification of Murine Granulocyte-Macrophage Progenitor Cells(CFU-GM) Using Counterflow Centrifugal Elutriation (S. Cooper &H. Broxmeyer).

OTHER SYSTEMS AND ASSAYS.

Culture of Human and Mouse Bone Marrow Stromal Cells (S. Perkins& R. Fleischman).

List of Suppliers.

Index.

Subject Areas: Biology, life sciences [PS]