Culture Media, Solutions, and Systems in Human ART

Detailed discussion of the history, current status and significance of ART media and the culture systems for their use.

Patrick Quinn (Edited by)

9781107619531, Cambridge University Press

Paperback / softback, published 27 March 2014

296 pages, 56 b/w illus. 6 colour illus. 19 tables
23.3 x 15.8 x 1.5 cm, 0.52 kg

This volume describes culture media and solutions used in human ART; how they have been developed for in vitro human pre-implantation embryo development, the function and importance of the various components in media and solutions and how they interact, and how the systems in which these are used can influence outcomes. Chapters discuss inorganic solutes, energy substrates, amino acids, macromolecules, cytokines, growth factors, buffers, pH, osmolality, and the interaction of these parameters. The role of incubators and other physical factors are reviewed, along with the relevance and prospects of emerging technologies: morphokinetic analysis using time-lapse imaging and dynamic fluid incubation systems. Results of prospective randomized trials are emphasized to ascertain the added value of these techniques for selecting viable embryos. This comprehensive guide will be invaluable for embryologists, physicians and all personnel involved in the fluid products used in human ART seeking to optimize their successful use of these components.

Foreword Alan Trounson
Part I. Culture Media and Solutions: 1. Overview Patrick Quinn
2. Media and embryo interactions Patrick Quinn
3. Female tract environment and its relationship to ART media composition Henry J. Leese and Sarah Louise Whitear
4. Buffering systems in IVF Natalie A. Clark and Jason E. Swain
5. Essential features in media development for spermatozoa, oocytes and embryos David Mortimer
6. Macromolecular supplementation of embryo culture media Thomas B. Pool
7. Monozygotic twinning and assisted reproduction. Laboratory or clinical? Marius Meintjes
8. Amino acids and ammonium Deirdre Zander-Fox and Michelle Lane
9. Growth factors and cytokines in embryo development Sarah A. Robertson and Jeremy G. Thompson
10. Osmolality Jay M. Baltz
11. pH control in the embryo culture environment Joe Conaghan
12. In vitro maturation of immature human oocytes Ri-Cheng Chian, Shan-Jun Dai and Yao Wang
13. Oocyte and embryo cryopreservation media Diana Patricia Bernal, Ching-Chien Chang and Zsolt Peter Nagy
14. International regulation of ART media Theresa Jeary
Part II. Culture Systems: 15. Culture of embryos in dynamic fluid environments André Monteiro da Rocha and Gary Daniel Smith
16. Time-lapse imaging of embryo development: using morphokinetic analysis to select viable embryos Markus H. M. Montag, Kamilla S. Pedersen and Niels B. Ramsing
17. Optimizing culture conditions Kathleen A. Miller
18. Low cost IVF Luca Gianaroli, M. Cristina Magli and Anna P. Ferraretti
19. Incubators old and new Eduardo Kelly and Takeo Cho
20. Incubator management William R. Boone and H. Lee Higdon, III
21. Summary and conclusions Patrick Quinn
Index.

Subject Areas: Surgery [MN], Gynaecology & obstetrics [MJT]