Cryo-EM, Part C
Analyses, Interpretation, and Case Studies

This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM

Grant Jensen (Volume editor)

9780123849939, Elsevier Science

Hardback, published 23 November 2010

380 pages
22.9 x 15.1 x 2.6 cm, 0.84 kg

This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

1. Analyses of Subnanometer Resolution Cryo-EM Density Maps
Matthew L. Baker, Mariah R. Baker Corey F. Hryc Frank DiMaio
2. Methods for Segmentation and interpretation of cryo-EM data
Niels Volkmann
3. Integration of Cryo-EM with atomic and protein: protein interaction data
Friedrich Forster
4. Unified Data Resource for CryoEM
Catherine L. Lawson
5. Electron crystallography and aquaporins
Thomas Walz, Andreas D. Schenk, Richard K. Hite, Andreas Engel, Yoshinori Fujiyoshi
6. Cryo-Electron Microscopy Applications in the Study of Tubulin Structure, Microtubule Architecture, Dynamics and Assemblies, and Interaction of Microtubules with Motors
Kenneth H. Downin and Eva Nogales
7. Helical crystallization of two example membrane proteins: MsbA and the Ca2+-ATPase
Howard S. Young, John Paul Glaves, Lauren Fisher, Andrew Ward
8. Multi-particle cryo-EM of ribosomes
Christian M.T. Spahn, Justus Loerke, Jan Giesebrecht
9. Single-particle electron microscopy of animal fatty acid synthase: Describing macromolecular rearrangements that enable catalysis
Francisco Asturias, Edward J. Brignole
10. Tomography of actin cytoskeletal networks
Dorit Hanein
11. Visual Proteomics
Friedrich Förster, Bong-Gyoon Han, and Martin Beck
12. Cryo-Electron Tomography of Eukaryotic Cells
Ohad Medalia, Asaf Mader, Nadav Elad
13. 3d Visualization Of Hiv Virions By Cryo Electron Tomography
Jun Liu, Elizabeth R. Wright, Hanspeter Winkler
14. Automation in Single-Particle Electron Microscopy: Connecting the Pieces
Bridget Carragher, Dmitry Lyumkis, Arne Moeller, Anchi Cheng, Amber Herold, Eric Hou, Christopher Irving, Erica L. Jacovetty, Pick-Wei Lau, Anke Mulder, James Pulokas, Joel D. Quispe, Neil R. Voss, Clinton S. Potter

Subject Areas: Molecular biology [PSD], Biochemistry [PSB], Biophysics [PHVN], Microscopy [PDND], Science: general issues [PD]