• Cryo-EM Part A: Sample Preparation and Data Collection (Hardback) 9780123749062
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Cryo-EM Part A: Sample Preparation and Data Collection

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM.

Grant Jensen (Volume editor)

9780123749062, Elsevier Science

Hardback, published 22 November 2010

464 pages
22.9 x 15.1 x 2.9 cm, 0.88 kg

Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

Historical perspective

3D reconstruction from electron micrographs: a personal account of its development

David DeRosier

  1. Preparation of 2-D crystals

    2. Henning Stahlberg

  2. Helical Crystallization of Soluble and Membrane Binding Proteins

    3. Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie

  3. Plunge Freezing for Electron Cryomicroscopy

    4. Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen

  4. A Practical Guide to the Use of Monolayer Purification and Affinity Grids

    5. Thomas Walz, Deborah F. Kelly, Danijela Dukovski

  5. GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM

    6. Holger Stark

  6. Cryo-negative staining of macromolecular assemblies

    7. Sacha De Carlo, Holger Stark

  7. Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM

    8. Fred J. Sigworth, Liguo Wang

  8. Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing

    9. Mark S. Ladinsky

  9. Site-Specific Biomolecule Labeling with Gold Clusters

    10. Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld

  10. How to Operate a Cryo-Electron Microscope

    11. Huilin Li, Jingchuan Sun

  11. Collecting electron crystallographic data of two-dimensional protein crystals

    12. Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng

  12. Automated Data Collection for Electron Microscopic Tomography

    13. David Agard, Shawn Q. Zheng, J. W. Sedat

  13. Correlated Light and Electron Cryomicroscopy

    14. Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jürgen M. Plitzkod, Cindi L. Schwartz

  14. Phase Plates for Transmission Electron Microscopy

    15. Radostin Danev, Kuniaki Nagayama

  15. Radiation damage in electron cryomicroscopy

John L. Rubinstein, Lindsay A. Baker

Subject Areas: Molecular biology [PSD], Biochemistry [PSB], Biophysics [PHVN], Microscopy [PDND], Science: general issues [PD]
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