CRISPR Genome Surgery in Stem Cells and Disease Tissues

A cutting-edge resource for scientists and medical subspecialists involved in stem cell engineering and gene editing

Stephen H. Tsang (Edited by)

9780128178768, Elsevier Science

Paperback, published 19 October 2021

136 pages, 25 illustrations (15 in full color)
23.5 x 19 x 1 cm, 0.29 kg

CRISPR Genome Surgery in Stem Cells and Disease Tissues focuses uniquely on the clinical applications of CRISPR/Cas9 based technology. Topics include the latest advances in gene editing and its translational applications to various diseases, including retinal degenerative disease, recessively inherited diseases, and dominantly inherited diseases, to name a few. The book's target audience includes researchers, students, clinicians and the general public. This space that is not currently served by any existing resource, so this publication fills a gap in current literature.

1. History of CRISPR 2. Different Kinds of CRISPR (SpCas9, CjCas9, CaCas9, etc.) 3. Genome engineering in iPSC cells 4. CRISPR manipulations in stem cell lines for neurological diseases 5. Gene editing for the cornea 6. CRISPR screening for eye diseases 7. Employing Non-homologous End Joining and Homology-directed Repair for Treatment of Leber Congenital Amaurosis and Retinal Degeneration 8. Ethics of Gene Editing in Human Stem Cells 9. sGRNA 10. CRISPR vs ZFN/TALEN

Subject Areas: Cellular biology [cytology PSF]