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Computational Biology
A Hypertextbook

Scott T. Kelley (Author), Dennis Didulo (Author)

9781683670025, Wiley

Paperback / softback, published 1 January 2018

210 pages
25.2 x 17.8 x 1 cm, 0.522 kg

This textbook is for anyone who needs to learn the basics of bioinformatics—the use of computational methods to better understand biological systems. Computational Biology covers the principles and applications of the computational methods used to study DNA, RNA, and proteins, including using biological databases such as NCBI and UniProt; performing BLAST, sequence alignments, and structural predictions; and creating phylogenetic trees. It includes a primer that can be used as a jumping off point for learning computer programming for bioinformatics.

This text can be used as a self-study guide, as a course focused on computational methods in biology/bioinformatics, or to supplement general courses that touch on topics included within the book. Computational Biology's robust interactive online components “gamify” the study of bioinformatics, allowing the reader to practice randomly generated problems on their own time to build confidence and skill and gain practical real-world experience. The online component also assures that the content being taught is up to date and accurately reflects the ever-changing landscape of bioinformatics web-based programs.

Preface ix

For the Instructor xi

For the Student xiii

Acknowledgments xiv

About the Authors xv

Chapter –1 Getting Started 1

Chapter 00 Introduction 5

Activity 0.1: Biological Databases and Data Storage 20

Chapter 01 BLAST 31

Activity 1.1: BLAST Algorithm 36

Chapter 02 Protein Analysis 47

Activity 2.1: Hydrophobicity Plotting 52

Activity 2.2: Protein Secondary Structure Prediction 58

Chapter 03 Sequence Alignment 67

Activity 3.1: Dynamic Programming 74

Chapter 04 Patterns in the Data 91

Activity 4.1: Protein Sequence Motifs 94

Activity 4.2: Position-Specific Weight Matrices 102

Chapter 05 RNA Structure Prediction 111

Activity 5.1: RNA Structure Prediction 118

Chapter 06 Phylogenetics 133

Activity 6.1: Phylogenetic Analysis 140

Chapter 07 Probability: All Mutations are not Equal (-ly Probable) 157

Activity 7.1: Generating PAM and BLOSUM Substitution Matrices 163

Chapter 08 Bioinformatics Programming: A Primer 179

Index 191

Subject Areas: Biology, life sciences [PS]

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