Coenzyme B12 Enzymes Part A

Edited by some of the best scientists in the field, this series presents the latest updates in the field of enzymology

Neil Marsh (Volume editor)

9780323915915

Hardback, published 19 May 2022

390 pages
22.9 x 15.2 x 2.6 cm, 0.77 kg

B12 Enzyme, Volume 667 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting timely, interesting chapters, including Part I: B12 Biosynthesis and B12 Chaperones, Probing the preference and regioselectivity of lower ligand activation and its role in cobamide diversity, Biosynthesis of corrinoids, The human B12 trafficking chaperones: CblC, CblD and ATR, A method for the efficient adenosylation of corrinoids, Production, purification and liposome reconstitution of cobamide synthase, Extraction and Purification of Cobamides from Bacterial Cultures, Part II: The many faces of B12-dependent enzyme reactions, Methylcobalamin-dependent methionine synthase, and much more.

1. B₁₂ Biosynthesis and B₁₂ Chaperones Martin Warren University of Kent, U.K. 2. Biosynthesis of corrinoids Amrita Hazra IISER Pune, India 3. Guardian of cobamide diversity: probing the role of CobT in lower ligand activation in the biosynthesis of vitamin B₁₂ and other cobamide cofactors Michiko Taga University of California, Berkeley 4. Purification and Detection of Vitamin B₁₂ Analogs Jorge Escalante-Semerena University of Georgia 5. A method for the efficient adenosylation of corrinoids Jorge Escalante-Semerena University of Georgia 6. A method for the production, purification and liposome reconstitution of cobamide synthase Jorge Escalante-Semerena University of Georgia 7. A method for the Isolation of  -ribazole from Vitamin B12, and its Enzymatic Conversion to -ribazole 5’-phosphate Ruma Banerjee University of Michigan 8. The human B₁₂ trafficking chaperones: CblC, CblD and ATR Bernhard Kräutler University of Innsbruck, Austria 9. Synthesis and applications of "antivitamin" B₁₂ derivatives B₁₂-dependent enzyme reactions Tetsuo Toraya Okayama University, Japan 10. Coenzyme B₁₂-dependent eliminases: Diol and glycerol dehydratases and ethanolamine ammonia-lyase Tetsuo Toraya Okayama University, Japan 11. Reactivating chaperones for coenzyme B₁₂-dependent eliminases: Diol and glycerol dehydratases and ethanolamine ammonia-lyase Wolfgang Buckel Marburg University 12. Glutamate mutase and 2-methyleneglutarate mutase Ruma Banerjee University of Michigan 13. Human B₁₂ dependent enzymes: Methionine synthase and methylmalonyl-CoA mutase David Leys Manchester University 14. Heterologous production and biophysical characterisation of catabolic Nitratireductor pacificus pht-3B reductive dehalogenase Nigel Scrutton Manchester University 15. An unusual light-sensing function for coenzyme B₁₂ in bacterial transcription regulator CarH

Subject Areas: Molecular biology [PSD], Biochemistry [PSB]