Cilia: From Mechanisms to Disease–Part B

Offers a detailed overview of the protocols used to study cilia structure and various aspects of ciliary functions

Jose Manuel Bravo-San Pedro (Volume editor), Lorenzo Galluzzi (Volume editor)

9780443185885, Elsevier Science

Hardback, published 21 April 2023

270 pages
23.4 x 19 x 2.3 cm, 0.45 kg

Cilia: From Mechanisms to Disease Part B, Volume 176 of the Methods in Cell Biology series, presents interesting chapters on topics such as The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish, Human LUHMES and NES cells as models for studying primary cilia in neurons, Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations, Single-molecule imaging in the primary cilium, Methods to analyze primary cilia in mouse cardiac lesion model, Methods to study motile ciliated cell types in a zebrafish brain mode, and more.

1. The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish
Magdalena Cardenas Rodriguez and Iain Drummond
2. Human LUHMES and NES cells as models for studying primary cilia in neurons
Peter Swoboda
3. Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations
Russell J. Ferland
4. Single-molecule imaging in the primary cilium
Lucien E. Weiss
5. Methods to analyze primary cilia in mouse cardiac lesion model
Alfredo Criollo Sr., Daniel Peña-Oyarzun, Rodrigo Troncoso, Joseph A. Hill, Sergio Lavandero and Mauricio Budini Sr.
6. Methods to study motile ciliated cell types in a zebrafish brain mode
Nathalie Jurisch-Yaksi
7. Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome)
Diana Valverde
8. Modelling ciliopathies in patient-derived primary cells
Markus Schueler and Kai-Uwe Eckardt
9. Analysis of motility and mucociliary function of tracheal epithelial cilia
Koji Ikegami
10. High-speed Super-resolution SPEED Microscopy to study Live Primary Cilium
Weidong Yang
11. Method for siRNA delivery in retina explants
Florent Poulhès
12. Methods to study primary cilia and autophagy in the brain
Olatz Pampliega
13. Multi-color live-cell fluorescence imaging of primary ciliary membrane assembly dynamics
Christopher Westlake

Subject Areas: Cellular biology [cytology PSF], Molecular biology [PSD], Developmental biology [PSC]