Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems

This new volume in the Methods in Enzymology series focuses on chemical tools for imaging, manipulating and tracking biological systems

David M. Chenoweth (Volume editor)

9780128201459, Elsevier Science

Hardback, published 15 May 2020

358 pages
22.9 x 15.1 x 2.5 cm, 0.7 kg

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Prokaryotic and Eukaryotic Systems, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more.

1. Utility of bacterial peptidoglycan recycling enzymes in the chemoenzymatic synthesis of valuable UDP sugar substrates

Ophelia I. Ukaegbu, Kristen E. DeMeester, Hai Liang, Ashley R. Brown, Zachary S. Jones and Catherine Leimkuhler Grimes

2. Chemical tools for selective activity profiling of bacterial penicillin-binding proteins

Shabnam Sharifzadeh, Nathaniel W. Brown, Joshua D. Shirley, Kevin E. Bruce, Malcolm E. Winkler and Erin E. Carlson

3. Fluorescent stem peptide mimics: In situ probes for peptidoglycan crosslinking

Samir Gautam, Taehan Kim, Rebecca Howell and David A. Spiegel

4. Gram-scale preparation of the antibiotic lead compound salicyl-AMS, a potent inhibitor of bacterial salicylate adenylation enzymes

Nihar Kinarivala, Lisa Standke, Tezcan Guney, Cheng Ji, Naoyoshi Noguchi, Yasutomi Asano and Derek S. Tan

5. Probe-enabled approaches for function-dependent cell sorting and characterization of microbiome subpopulations

Andrea K. Steiger, Sarah J. Fansler, Christopher Whidbey and Aaron T. Wright

6. Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activity

Byungchul Kim, Juliel Espinosa and Howard C. Hang

7. Controlled release of bioactive signaling molecules using DNA-icosahedron

Maulik S. Jani, Aneesh T. Veetil and Yamuna Krishnan

8. Imaging nascent transcription in wholemount vertebrate embryos to characterize zygotic genome activation

Hui Chen and Matthew C. Good

9. Engineering reversible cell-cell interactions with chemical biology

Clifford M. Csizmar and Carston R. Wagner

10. Fast phosphine-activated control of protein function using unnatural lysine analogues

Joshua S. Wesalo and Alexander Deiters

11. Photopharmacological control of lipid function

Johannes Morstein and Dirk Trauner

12. Expanding the substrate selectivity in SNAP/CLIP-tagging of intracellular targets

Miguel Macias-Contreras, Kevin N. Little and Lei Zhu

13. Light-induced protein proximity by activation of gibberellic acid derivatives in living cells

Michael J. Ziegler and Richard Wombacher

14. Photoactivatable trimethoprim-based probes for spatiotemporal control of biological processes

Daniel Z. Wu, Michael A. Lampson and David M. Chenoweth

15. Site-specific antibody fragment conjugates for targeted imaging

Robert Maloney, Zakey Yusuf Buuh, Yue Zhao and Rongsheng E. Wang

16. Quantifying protein-protein interactions of the acyl carrier protein with solvatochromic probes

Subject Areas: Molecular biology [PSD], Biochemistry [PSB]