Section 1: Tissue decellularization and characterization methods 1. Decellularization of porcine heart tissue to obtain extracellular matrix based hydrogels Rabeil Sakina, Aida Llucià-Valldeperas, Ana Henriques Lourenço, Abhishek Harichandan, Sandro Gelsomino, Paul Wieringa, Carlos Mota and Lorenzo Moroni 2. Method to decellularize the human chorion membrane Laura P. Frazão, Joana Vieira-de-Castro, Cristina Nogueira-Silva and Nuno M. Neves 3. Protocols for decellularization of human amniotic membrane Sadjad Khosravimelal, Maryam Momeni, Mahdieh Gholipur, Subhas C. Kundu and Mazaher Gholipourmalekabadi 4. Use of supercritical CO2 in soft tissue decellularization Bengisu Topuz, Gülçin Günal, Selcan Guler and Halil Murat Aydin 5. Corneal extracellular matrix decellularization Mark Ahearne

Section 2: Decellularized tissues for tissue engineering and disease modelling 6. Implementation of a basement membrane invasion assay using mesenteric tissue Ritobrata Ghose, Alistair J. Rice, Ernesto Cortes, Upamanyu Ghose, Dariusz Lachowski and Armando del Rio Hernandez 7. Decellularized human bone as a 3D model to study skeletal progenitor cells in a natural environment Ana Rita Pereira, Maximilian Rudert and Marietta Herrmann 8. Cell sheet tissue engineering for scaffold-free three-dimensional (3D) tissue reconstruction Kyungsook Kim, Sophia Bou-Ghannam and Teruo Okano 9. Decellularized matrices for tumor cell modeling Virginia Brancato, Maurizio Ventre, Rui L. Reis and Paolo Antonio Netti 10. Tissue-derived decellularized extracellular matrices toward cartilage repair and regeneration Piyali Das, Yogendra Pratap Singh, Biman B. Mandal and Samit Kumar Nandi

Section 3: Hybrid systems 11.The effect of aligned electrospun fibers and macromolecular crowding in tenocyte culture Dimitrios Tsiapalis, Shubhasmin Rana, Meletios Doulgkeroglou, Stephen Kearns, Jack Kelly, Yves Bayon and Dimitrios I. Zeugolis