Cell Biology Assays
Essential Methods

Comprehensive guide to essential methods in cell biology

Julio E. Celis (Editor-in-chief), Fanny Jaulin (Edited by), Cedric Espenel (Edited by)

9780123751522, Elsevier Science

Paperback / softback, published 17 November 2009

298 pages
23.4 x 19 x 2 cm, 0.63 kg

This text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems.

1. Permeabilized Epithelial Cells to Study Exocytic Membrane Transport
2. Studying Excit and Surface Delivery of Post-Golgi Transport Intermediates Using in vitro and Live-Cell Microscopy-Based Approaches
3. Assays Measuring Membrane Transport in the Endocytic Pathway
4. Microsome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport In Mammalian Cells
5. Cotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells
6. Use of Permeabilsed Mast Cells to Analyze Regulated Exocytosis
7. Syringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell Loading
8. Cell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial Monolayers
9. Protein Translocation into Mitochondria
10. Polarographic Assays of Mitochrondrial Functions
11. Analysis of Nuclear Protein Import and Export in Digitonin-Permeabilized Cells
12. Heterokaryons: An Assay for Nucleocytoplasmic Shuttling
13. DNA Replication-Dependent Chromatin Assembly System
14. Cygnets: Intracellular Guanosine 3’,5-Cycli Monophosphate Sensing in Primary Cells Using Fluorescence Energy Transfer
15. Ca2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos)
16. Ratiometric Pericam
17. Fuorescent Indicators for Imaging Protein Phosphorylation in Single Living Cells
18. In Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32P Labeling of Cellular Proteins
19. Dissecting Pathways
    in Situ Electroporation for the Study of Signal Transduction and Gap Junctional Communication
20. Detection of Protein-Protein Interactions in vivo Using Cyan and Yellow Fluorescent Proteins
21. Tracking Individual Chromosomes with Integrated Arrays of Iacop Sites and GFP-laci Repressor: Analyzing Position and Dynamics of Chromosomal Loci in Saccharomyces cerevisiae
22. Microtubule Motility Assays
23. In vitro Assays for Mitotic Spindle Assembly and Function
24. In vitro Motility Assays with Actin
25. Use of Brain Cytosolic Extracts for Studying Actin-Based Motility of Listeria monocytogenes
26. Pedestal Formation by Pathogenic Escherichia coli: A Model System for Studying Signal Transduction to the Actin Cytoskeleton
27. Listeria monocytogenes: Techniques to Analyze Bacterial Infection in vitro
28. Measurement of Cellular Contractile Forces Using Patterned Elastomer

Subject Areas: Cellular biology [cytology PSF]