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Cell Biological Applications of Confocal Microscopy

Brian Matsumoto (Volume editor)

9780124802773

Hardback, published 4 January 2003

520 pages
23.4 x 19 x 3.1 cm, 1.35 kg

Praise for the Series: "The series is invaluable for workers at all levels of cell biology." --Nature

Praise for the first edition: "The diverse applications presented in this book reflect the creativity and technical skills of the many contributors while emphasizing the power and promise of CM." --Trends in Cell Biology

This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities.

An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM).

Introduction to Confocal Microscopy.
Direct-View High-Speed Confocal Scanner - the CSU-10; Introduction to Two-Photon Microscopes.
Confocal Microscopy: Important Considerations for Accurate Imaging.
Multicolor-Laser Scanning Confocal Immunofluorescence Microscopy:
Practical Application and Limitations.
Practical Aspects of Objective Lens Selection for Confocal and Multi-Photon Digital Imaging Techniques.
Resolution of Subcellular Detail in Thick Tissue Sections: Immunohistochemical Preparation and Fluorescence Confocal Microscopy.
Confocal Fluorescence Microscopy for Tetrahymena. Confocal Imaging for Drosophila Embryos.
Confocal Fluorescence Microscopy of the Cytoskeleton of Amphibian Oocytes and Embryos. Confocal Fluorescence Microscopy Measurements of pH and Calcium in Living Cells.
Confocal and Non-Linear Optical Imaging of Potentiometric Dyes.
Measurement and Intracellular Ca2+ Concentration. Running and Setting Up a Confocal Microscope Core Facility.

Subject Areas: Cellular biology [cytology PSF], Molecular biology [PSD], Developmental biology [PSC], Life sciences: general issues [PSA], Biology, life sciences [PS], Biophysics [PHVN]

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