Calculations for Molecular Biology and Biotechnology
A Guide to Mathematics in the Laboratory

A must have guide covering calculations in Molecular Biology and Biotechnology

Frank H. Stephenson (Author)

9780123756909

Paperback / softback, published 30 July 2010

460 pages
23.4 x 19 x 2.9 cm, 0.8 kg

Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing.

1. Scientific Notation and Metric Prefixes2. Solutions, Mixtures, and Media3. Cell Growth4. Working with Bacteriophage5. Quantitation of Nucleic Acids6. Labeling Nucleic Acids with Radioisotopes7. Oligonucleotide Synthesis8. The Polymerase Chain Reaction9. Real Time PCR10. Recombinant DNA11. Protein12. Centrifugation13. Forensic Science

Subject Areas: Biotechnology [TCB], Maths for engineers [TBJ], Molecular biology [PSD], Medical bioinformatics [MBF]