Biophysical Labeling Methods in Molecular Biology

This 1993 volume covers all aspects of the field of physical labels - the theoretical bases, the experimental techniques - and shows how to interpret the resulting data.

Gertz I. Likhtenshtein (Author)

9780521431323, Cambridge University Press

Hardback, published 26 March 1993

320 pages, 4 b/w illus. 5 tables
23.4 x 15.6 x 1.9 cm, 0.63 kg

"...should be of use to graduate students and those considering research in biophysical chemistry who want an historical overview of labeling methods." Bruce Eaton, Journal of the American Chemical Society

Physical labels such as stable nitroxide radicals, luminescent and photochromic chromophores, so-called Mössbauer atoms and electron-dense assemblies of heavy atoms have proved to be effective tools in solving many problems at the molecular level in biological systems. These physical labels are used as 'molecular rulers' to measure the distances between chosen groups and to measure the size, form and microrelief of objects. By providing information about these factors, the label provides information that can help the scientist to understand the structure of membranes, nucleic acids, enzymes and proteins and how they function. This 1993 volume covers all aspects of this field: the theoretical bases, the experimental techniques, and it also shows how to interpret the resulting data. It also critically discusses some recent results obtained with these techniques and gives an analysis of likely developments in the future.

1. The methods of spin labeling
2. Double labeling techniques
3. Fluorescent labeling methods
4. Triplet labeling methods
5. Mössbauer, electron-scattering, and other labeling methods
6. Studies of proteins and enzymes: structure, dynamics and mechanism of action
7. Structure and dynamics of membrane
8. Nucleic acids and other biosystems, biological assays
9. Conclusion
References
Subject index.

Subject Areas: Molecular biology [PSD], Scientific equipment, experiments & techniques [PDN]