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Biological Applications of Infrared Spectroscopy

Barbara H. Stuart (Author), David J. Ando (Edited by)

9780471974147, Wiley

Paperback / softback, published 18 July 1997

266 pages
22.8 x 15.2 x 1.6 cm, 0.312 kg

Diese Einführung in die Grundlagen der Infrarot-Spektroskopie ist besonders für Biologen und Biochemiker gedacht. Von den theoretischen Grundlagen über experimentelle Methoden und instrumentelle Ausrüstung bis hin zur Interpretation von Spektren biologisch relevanter Spezies (Proteine, Peptide, Lipide, Nucleinsäuren, Membranen) wird das Gebiet umfassend und auch für Nichtspezialisten verständlich behandelt.

Aus dem Inhalt:
Introduction: Electromagnetic Radiation

Distribution of Lifetimes of Energy States

Molecular Vibrations
Instrumentation: Representation of Spectra

Dispersive Spectrometers

Fourier Transform Spectrometers
Sampling Methods: Transmission methods

Reflectance Methods

Microsampling Methods

Gas Chromatography

Temperature Studies

Photoacoustic Spectroscopy
Spectrum Interpretation: Group Frequencies

Summary of Characteristic Frequencies

Aldehydes and Ketones

Complicating Factors

Interpretation of Unknown Compounds
Quantitative Analysis: Spectrum Manipulation

Pathlength Calibration

Determination of Concentration

Simple Analysis

Multicomponent Analysis
Applications: Proteins and Peptides

Lipids

Biomembranes

Animal Tissue

Carbohydrates

Pharmaceuticals

Foods

Plant Material.

Subject Areas: Chemistry [PN]

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