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Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls
A step-by-step instruction guide for researchers.
John T. Corthell (Author)
9780128014615, Elsevier Science
Paperback, published 16 April 2014
124 pages
22.9 x 15.1 x 1 cm, 0.18 kg
Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials.
Introduction General Notes DNA and RNA Extraction Protocols Agarose Gell Electrophoresis Reverse Transcription (RT) and Polymerase Chain Reaction (PCR) Antibodies and Titrations Protein Extraction Immunoblotting (Western Blot) Immunoprecipitation Perfusion and Immersion Fixation Immunohistochemistry In situ Hybridization References
Subject Areas: Neurosciences [PSAN]
