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Basic Biotechniques for Bioprocess and Bioentrepreneurship
A practical, comprehensive laboratory manual focusing on basic techniques in the laboratory during bioprocess development, commercialization and technology transfer
Arvind Kumar Bhatt (Edited by), Ravi Kant Bhatia (Edited by), Tek Chand Bhalla (Edited by)
9780128161098, Elsevier Science
Paperback, published 6 February 2023
518 pages
27.6 x 21.6 x 3.2 cm, 0.45 kg
Basic Biotechniques for Bioprocess and Bioentrepreneurship deals with the entire field of industrial biotechnology, starting from the basic laboratory techniques to scale-up, process development, demonstration, and finally its commercialization. The book compiles currently scattered materials on this topic and updates this information based on practical experience and requirements. The book will be an ideal source for new entrepreneurs who wish to start their own commercial units.
Part I: Isolation, screening and culture maintenance 1. Isolation of microorganisms 2. Screening strategies 3. Identification, morphological, biochemical, and genetic characterization of microorganisms 4. Microbial activity and productivity enhancement strategies 5. Culture maintenance, preservation, and strain improvement Part II: Laboratory techniques and instrumentation 6. Biomolecules: Types, homogenization, bead beater, sonication 7. Centrifugation: Basic principle, types 8. Spectroscopy—Principle, types, and applications 9. Protein purification: Basic principles and techniques 10. Chromatography: Basic principle, types, and applications 11. Electrophoresis: Basic principle, types, and applications Part III: Genomic and proteomic analysis 12. DNA, RNA isolation, primer designing, sequence submission, and phylogenetic analysis 13. RDT and genetic engineering: Basic of RDT method, PCR, and application 14. Protein sequence analysis 15. Computational strategies and tools for protein tertiary structure prediction 16. Docking strategies 17. A beginner’s guide to measuring binding affinity during biomolecular interactions Part IV: Industrially important enzymes 18. Enzymes and their significance in the industrial bioprocesses 19. Enzyme kinetics: Industrially important enzymes 20. Industrial enzymes: Basic information, assay, and applications Part V: Techniques in process development 21. Fundamentals of fermentation technology 22. Sterilization in bioprocesses 23. Scale-up: Lab to commercial scale 24. Bioprocess: Control, management, and biosafety issues 25. Demonstration and industrial scale-up 26. Downstream processing of biotechnology products 27. Waste management and environment Part VI: Feasibility, marketing and biobusiness 28. Biobusiness opportunities 29. Feasibility analysis and business plan 30. Patenting, IPR, and regulatory issues 31. Commercialization and technology transfers of bioprocess 32. Bioproduct marketing strategies
