Angiogenesis: In Vitro Systems

Angiogenesis has been identified as a “common denominator” in society’s most serious diseases and more than $4 billion has been invested in the research and development of angiogenesis-based medicines, making it one of the most heavily funded areas of medical research in history.

David A. Cheresh (Edited by)

9780123743152, Elsevier Science

Hardback, published 14 October 2008

432 pages
22.9 x 15.1 x 2.8 cm, 0.78 kg

Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over antiogenesis. Diseases that are angiogensis-dependent result when blood vessels either grow excessively or insufficiently.

Studying cell signaling pathways
Flow mediated signaling
Endothelial cell adhesion and migration on the ECM
EC sprouting assays
Lumen formation assays
Isolation and propagation of EC's or SMC's
Cell-based assays to screen anti-angiogenic agents (chemical approach
ES cell models of vascular network formation
Co-culture studies with EC plus other cell types
Aortic ring sprouting (multiple cell types present)
Effects of hypoxia or hyperoxia
Vascular Proteomics
In vitro vascular barrier function
EC leukocyte interactions
ECM signaling to the vasculature
Vascular Integrin signaling
Mechanical Signaling in blood vessels
Vascular and neuronal patterning
Vascular growth factors and their signals
VEGF receptor signal transduction
Development and testing of monoclonal antibodies directed to angiogenic targets.

Subject Areas: Biotechnology [TCB], Cellular biology [cytology PSF], Molecular biology [PSD], Biochemistry [PSB], Life sciences: general issues [PSA], Biophysics [PHVN], Cardiovascular medicine [MJD], Physiology [MFG]