A Practical Guide to the Study of Calcium in Living Cells

Leslie Wilson (Series edited by), Paul T. Matsudaira (Series edited by), Richard Nuccitelli (Volume editor)

9780125641418

Hardback, published 25 April 1994

384 pages
22.9 x 15.1 x 2.6 cm, 0.97 kg

"This multi-authored book... provides theoretical and applied aspects of different established techniques for the measurement of [Ca²⁺]i at submicromolar concentration. Each chapter is organized, keeping in view the needs of a person working in the laboratory, by giving the detailed methodology required for the implementation of that particular technique, and all the chapters are well referenced... The book provides a pragmatic approach for the estimation of cytosolic [Ca²⁺]i and also provides a means for studying the spatial and temporal resolution of [Ca²⁺]i. The book achieves the stated goal in the preface, 'this material will be very useful to the investigator who wants to apply any of these methods to his or her research.' It is a very well-written monograph and includes useful illustrations and helpful, practical points." --RAMESH BHALLA, University of Iowa, Iowa City

Approx.384 pages

Ca²⁺ Buffers and [Ca²⁺]I Perturbation Techniques:
D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca²⁺ Buffers.
R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca²⁺.
D. Thomas and M.R. Hanley, Pharmacological Tools for Perturbing Intracellular Calcium Storage.
Microelectrode Techniques for Measuring [Ca²⁺]I and Ca²⁺ Fluxes:
S. Baudet, L. Hove-Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes.
P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca²⁺ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca²⁺ Influx and Efflux.
C.A. Leech and G.G. Holz, IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels.
Fluorescence Techniques for Imaging [Ca²⁺]I:
J.P.Y. Kao, Practical Aspects of Measuring [Ca²⁺] with Fluorescent Indicators.
S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH.
T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques.
P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca²⁺ in Cells.
S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes.
Use of Aequorin for [Ca²⁺]I Imaging:
K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca²+]I Changes Using a Photomultiplier Tube.
A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca²⁺]I with Aequorin Using a Photon Imaging Detector.
R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles.
Index.

Subject Areas: Natural history [WN], Cellular biology [cytology PSF], Developmental biology [PSC], Neurosciences [PSAN], Life sciences: general issues [PSA], Biology, life sciences [PS]