A Practical Guide to Basic Laboratory Andrology

A practical, extensively illustrated, fully updated manual covering procedures for human semen analysis and sperm functional assessment.

David Mortimer (Author), Lars Björndahl (Author), Christopher L. R. Barratt (Author), José Antonio Castilla (Author), Roelof Menkveld (Author), Ulrik Kvist (Author), Juan G. Alvarez (Author), Trine B. Haugen (Author)

9781009181631, Cambridge University Press

Paperback / softback, published 10 March 2022

380 pages
25.4 x 17.8 x 1.8 cm, 0.82 kg

This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes quality control considerations and the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. The interpretation of laboratory results in the clinical context is highlighted throughout, and safe laboratory practice is emphasized. Fully revised, incorporating the new ISO TS 23162 on basic human semen analysis throughout, this is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.

List of abbreviations:1. Introduction
2. Basic physiology
3. Basic semen examination
4. Extended semen analysis
5. Sperm DNA
6. Computer-aided sperm analysis
7. Sperm function tests
8. Tests of sperm-cervical mucus interaction
9. Sperm preparation
10. Sperm cryopreservation
11. Preparation of surgically retrieved spermatozoa
12. Quality management and accreditation
13. Risk management
14. Reproductive toxicology
15. Andrology laboratory safety
Appendices: 16. Interpreting andrology laboratory results#
17. Equipment required for a basic andrology laboratory
18. Home testing
19. Example andrology laboratory forms
19. Comparison of replicate counts
Index.

Subject Areas: Men's health [VFDM], Urology & urogenital medicine [MJS], Embryology [MFKC3], Infertility & fertilization [MFKC1]