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A Laboratory Guide to the Tight Junction
A comprehensive collection of experimental protocols to analyze paracellular channels in various cell, tissue, and animal models
Jianghui Hou (Author)
9780128186473
Paperback, published 28 April 2020
420 pages, 180 illustrations (100 in full color)
22.9 x 15.1 x 2.7 cm, 0.68 kg
A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases.
1. Introduction2. Biochemical approaches for tight junction3. Biophysical approaches for tight junction4. Histological approaches for tight junction5. Light microscopy for tight junction6. Electron microscopy for tight junction7. Cell models of tight junction biology8. Mouse models of tight junction physiology9. Perspective
Subject Areas: Cellular biology [cytology PSF], Biochemistry [PSB], Life sciences: general issues [PSA], Personal & public health [MBNH], Biotechnology industries [KNDH1]
