{"product_id":"zebrafish-methods-for-assessing-drug-safety-and-toxicity-hardback-9780470425138","title":"Zebrafish; Methods for Assessing Drug Safety and Toxicity (Hardback) 9780470425138","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eZebrafish\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eMethods for Assessing Drug Safety and Toxicity\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003ePatricia McGrath (Edited by), P McGrath (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780470425138, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 23 March 2012\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e368 pages\u003cbr\u003e23.4 x 15.8 x 2.5 cm, 0.658 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e“This book should prove a useful reference for students, researchers and biomedical science professionals working with this species as an evolving model for toxicity assessment.”  (\u003ci\u003eBritish Toxicology Society New\u003c\/i\u003e, 1 November 2012)\u003c\/p\u003e\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003ci\u003eZebrafish: Methods for Assessing Drug Safety and Toxicity\u003c\/i\u003e offers a practical guide for using zebrafish as a tool for toxicology studies.   \u003cp\u003eConsolidating key protocols and approaches to help researchers navigate the important and evolving field of zebrafish models for toxicity screening, this new title describes the methods for using the zebrafish as a model organism to assess compound-induced toxicity on all major organs.  Individual chapters that concentrate on assays for each organ system are included and various analytical tools including microscopy, microplate readers, high content imaging systems, ECG, blood pressure monitors, high speed video and motion detectors are described.\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePreface xi  \u003cp\u003eContributors xiii\u003c\/p\u003e \u003cp\u003eAcknowledgments xvii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1. The Reproductive Biology and Spawning of Zebrafish in Laboratory Settings 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 1\u003c\/p\u003e \u003cp\u003e1.2 Overview of Zebrafish Reproductive Biology and Behavior 2\u003c\/p\u003e \u003cp\u003e1.3 Spawning Techniques and Technology 5\u003c\/p\u003e \u003cp\u003e1.4 Determining Factors for Reproduction in Laboratory Stocks of Zebrafish 8\u003c\/p\u003e \u003cp\u003e1.5 Conclusions 11\u003c\/p\u003e \u003cp\u003eReferences 11\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2. Developmental Toxicity Assessment in Zebrafish 15\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 15\u003c\/p\u003e \u003cp\u003e2.2 Methods 16\u003c\/p\u003e \u003cp\u003e2.3 Results 20\u003c\/p\u003e \u003cp\u003e2.4 Discussion 23\u003c\/p\u003e \u003cp\u003eReferences 25\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3. Use of Emerging Models for Developmental Toxicity Testing 27\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Importance of Assessing Developmental Toxicity 27\u003c\/p\u003e \u003cp\u003e3.2 Current Methods for Assessing Developmental Toxicity 27\u003c\/p\u003e \u003cp\u003e3.3 Use of Emerging Models for Developmental Toxicity Testing 28\u003c\/p\u003e \u003cp\u003e3.4 New Guidelines for Chemical Testing Using Zebrafish 29\u003c\/p\u003e \u003cp\u003e3.5 Conclusions 42\u003c\/p\u003e \u003cp\u003eReferences 43\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4. Assessment of Drug-Induced Cardiotoxicity in Zebrafish 45\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 45\u003c\/p\u003e \u003cp\u003e4.2 Zebrafish Heart 45\u003c\/p\u003e \u003cp\u003e4.3 Summary of Cardiotoxicity Study Design and Results 47\u003c\/p\u003e \u003cp\u003e4.4 Materials and Methods 47\u003c\/p\u003e \u003cp\u003e4.5 Results 50\u003c\/p\u003e \u003cp\u003e4.6 Conclusions 54\u003c\/p\u003e \u003cp\u003eReferences 54\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5. Cardiotoxicity Studies in Zebrafish 55\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 55\u003c\/p\u003e \u003cp\u003e5.2 Repolarization Toxicity 56\u003c\/p\u003e \u003cp\u003e5.3 Initial Screening: Bradycardia 56\u003c\/p\u003e \u003cp\u003e5.4 High-Resolution Assays of Repolarization 59\u003c\/p\u003e \u003cp\u003e5.5 Future Directions 61\u003c\/p\u003e \u003cp\u003eReferences 62\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6. In Vivo Recording of the Adult Zebrafish Electrocardiogram 65\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 65\u003c\/p\u003e \u003cp\u003e6.2 Optimization of Zebrafish Electrocardiogram Recording 66\u003c\/p\u003e \u003cp\u003e6.3 Basic Intervals 68\u003c\/p\u003e \u003cp\u003e6.4 Drug Effects 69\u003c\/p\u003e \u003cp\u003e6.5 Conclusions 71\u003c\/p\u003e \u003cp\u003eReferences 72\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7. Hematopoietic and Vascular System Toxicity 73\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 73\u003c\/p\u003e \u003cp\u003e7.2 Hematopoiesis and Vascular Development in the Zebrafish 73\u003c\/p\u003e \u003cp\u003e7.3 Morphological and Functional Assays to Assess Toxicity 77\u003c\/p\u003e \u003cp\u003e7.4 Summary 85\u003c\/p\u003e \u003cp\u003eAcknowledgment 85\u003c\/p\u003e \u003cp\u003eReferences 85\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8. Hepatotoxicity Testing in Larval Zebrafish 89\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction: The Larval Zebrafish Model 89\u003c\/p\u003e \u003cp\u003e8.2 Liver Development 89\u003c\/p\u003e \u003cp\u003e8.3 Hepatic Gene Knockdown and Mutation 91\u003c\/p\u003e \u003cp\u003e8.4 Hepatotoxicity Testing in Drug Discovery 92\u003c\/p\u003e \u003cp\u003e8.5 Phenotypic-Based Larval Zebrafish Hepatotoxicity Screens 93\u003c\/p\u003e \u003cp\u003e8.6 Secondary and Mechanistic Liver Assays 96\u003c\/p\u003e \u003cp\u003e8.7 Conclusions 99\u003c\/p\u003e \u003cp\u003eReferences 99\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9. Whole Zebrafish Cytochrome P450 Assay for Assessing Drug Metabolism and Safety 103\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 103\u003c\/p\u003e \u003cp\u003e9.2 Background and Significance 104\u003c\/p\u003e \u003cp\u003e9.3 Materials and Methods 105\u003c\/p\u003e \u003cp\u003e9.4 Results 107\u003c\/p\u003e \u003cp\u003e9.5 Conclusions 113\u003c\/p\u003e \u003cp\u003eAcknowledgment 113\u003c\/p\u003e \u003cp\u003eReferences 113\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10. Methods for Assessing Neurotoxicity in Zebrafish 117\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 117\u003c\/p\u003e \u003cp\u003e10.2 Limitations of Current Neurotoxicity Testing 118\u003c\/p\u003e \u003cp\u003e10.3 Assessing Neurotoxicity in Zebrafish 118\u003c\/p\u003e \u003cp\u003e10.4 Summary 130\u003c\/p\u003e \u003cp\u003eAcknowledgments 131\u003c\/p\u003e \u003cp\u003eReferences 131\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11. Zebrafish: A Predictive Model for Assessing Cancer Drug-Induced Organ Toxicity 135\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 135\u003c\/p\u003e \u003cp\u003e11.2 Materials and Methods 136\u003c\/p\u003e \u003cp\u003e11.3 Results 139\u003c\/p\u003e \u003cp\u003e11.4 Conclusions 149\u003c\/p\u003e \u003cp\u003eReference 149\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12. Locomotion and Behavioral Toxicity in Larval Zebrafish: Background, Methods, and Data 151\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 151\u003c\/p\u003e \u003cp\u003e12.2 Background 152\u003c\/p\u003e \u003cp\u003e12.3 Locomotion 153\u003c\/p\u003e \u003cp\u003e12.4 Zebrafish Models 154\u003c\/p\u003e \u003cp\u003e12.5 Analyzing Larval Locomotion 155\u003c\/p\u003e \u003cp\u003e12.6 Chemical Effects on Larval Locomotion 158\u003c\/p\u003e \u003cp\u003e12.7 Conclusions 161\u003c\/p\u003e \u003cp\u003eAcknowledgments 162\u003c\/p\u003e \u003cp\u003eReferences 162\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13. Zebrafish: A Predictive Model for Assessing Seizure Liability 165\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 165\u003c\/p\u003e \u003cp\u003e13.2 Materials and Methods 167\u003c\/p\u003e \u003cp\u003e13.3 Results 171\u003c\/p\u003e \u003cp\u003e13.4 Conclusions 174\u003c\/p\u003e \u003cp\u003eReferences 175\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14. Zebrafish: A New In Vivo Model for Identifying P-Glycoprotein Efflux Modulators 177\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction 177\u003c\/p\u003e \u003cp\u003e14.2 Materials and Methods 179\u003c\/p\u003e \u003cp\u003e14.3 Results 182\u003c\/p\u003e \u003cp\u003e14.4 Conclusions 188\u003c\/p\u003e \u003cp\u003eAcknowledgment 188\u003c\/p\u003e \u003cp\u003eReferences 189\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15. Assessment of Effects on Visual Function in Larval Zebrafish 191\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction 191\u003c\/p\u003e \u003cp\u003e15.2 Development of Visual System in Zebrafish 192\u003c\/p\u003e \u003cp\u003e15.3 Methods for Assessing Visual Function in Larval Zebrafish 194\u003c\/p\u003e \u003cp\u003e15.4 Conclusions 202\u003c\/p\u003e \u003cp\u003eReferences 202\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16. Development of a Hypoxia-Induced Zebrafish Choroidal Neovascularization Model 205\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e16.1 Introduction 205\u003c\/p\u003e \u003cp\u003e16.2 Materials and Methods 207\u003c\/p\u003e \u003cp\u003e16.3 Results 210\u003c\/p\u003e \u003cp\u003e16.4 Discussion 215\u003c\/p\u003e \u003cp\u003eAcknowledgments 217\u003c\/p\u003e \u003cp\u003eReferences 217\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17. Zebrafish Xenotransplant Cancer Model for Drug Screening 219\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e17.1 Introduction 219\u003c\/p\u003e \u003cp\u003e17.2 Background and Significance 219\u003c\/p\u003e \u003cp\u003e17.3 Materials and Methods 221\u003c\/p\u003e \u003cp\u003e17.4 Results 226\u003c\/p\u003e \u003cp\u003e17.5 Conclusions 230\u003c\/p\u003e \u003cp\u003eReferences 231\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18. Zebrafish Assays for Identifying Potential Muscular Dystrophy Drug Candidates 233\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e18.1 Introduction 233\u003c\/p\u003e \u003cp\u003e18.2 Materials and Methods 238\u003c\/p\u003e \u003cp\u003e18.3 Results 242\u003c\/p\u003e \u003cp\u003e18.4 Discussion 251\u003c\/p\u003e \u003cp\u003eAcknowledgment 252\u003c\/p\u003e \u003cp\u003eReferences 252\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19. Cytoprotective Activities of Water-Soluble Fullerenes in Zebrafish Models 257\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e19.1 Introduction 257\u003c\/p\u003e \u003cp\u003e19.2 Materials and Methods 261\u003c\/p\u003e \u003cp\u003e19.3 Results 265\u003c\/p\u003e \u003cp\u003e19.4 Discussion 277\u003c\/p\u003e \u003cp\u003e19.5 Conclusions 278\u003c\/p\u003e \u003cp\u003eAcknowledgments 278\u003c\/p\u003e \u003cp\u003eReferences 279\u003c\/p\u003e \u003cp\u003e\u003cb\u003e20. Fishing to Design Inherently Safer Nanoparticles 283\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e20.1 Introduction 283\u003c\/p\u003e \u003cp\u003e20.2 Application of Embryonic Zebrafish 284\u003c\/p\u003e \u003cp\u003e20.3 Tier 1: Rapid Toxicity Screening 286\u003c\/p\u003e \u003cp\u003e20.4 Tier 2: Cellular Toxicity and Distribution 288\u003c\/p\u003e \u003cp\u003e20.5 Tier 3: Molecular Expression 289\u003c\/p\u003e \u003cp\u003e20.6 Embryonic Zebrafish Data to Design “Safer” Nanoparticles 291\u003c\/p\u003e \u003cp\u003e20.7 Conclusions 292\u003c\/p\u003e \u003cp\u003eReferences 292\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21. Radiation-Induced Toxicity and Radiation Response Modifiers in Zebrafish 295\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e21.1 Introduction 295\u003c\/p\u003e \u003cp\u003e21.2 Materials and Methods 296\u003c\/p\u003e \u003cp\u003e21.3 Validation of Zebrafish Embryos as a Model System for Radiation Protectors\/Sensitizers 298\u003c\/p\u003e \u003cp\u003e21.4 Gross Morphological Alterations Associated with Radiation Exposure 299\u003c\/p\u003e \u003cp\u003e21.5 Radiation-Associated Apoptosis Incidence 299\u003c\/p\u003e \u003cp\u003e21.6 Radiation-Associated Gastrointestinal Toxicity 300\u003c\/p\u003e \u003cp\u003e21.7 Radiation-Associated Nephrotoxicity 301\u003c\/p\u003e \u003cp\u003e21.8 Ototoxicity in Irradiated Zebrafish 301\u003c\/p\u003e \u003cp\u003e21.9 Radiation Protectors in Zebrafish 302\u003c\/p\u003e \u003cp\u003e21.10 Summary 303\u003c\/p\u003e \u003cp\u003eReferences 304\u003c\/p\u003e \u003cp\u003e\u003cb\u003e22. Caudal Fin Regeneration in Zebrafish 307\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e22.1 Introduction 307\u003c\/p\u003e \u003cp\u003e22.2 Signaling and Epimorphic Regeneration 308\u003c\/p\u003e \u003cp\u003e22.3 Caudal Fin Architecture 308\u003c\/p\u003e \u003cp\u003e22.4 Stages of Epimorphic Regeneration 309\u003c\/p\u003e \u003cp\u003e22.5 Methodology 311\u003c\/p\u003e \u003cp\u003e22.6 Strategies Used to Manipulate Gene Function During Fin Regeneration 312\u003c\/p\u003e \u003cp\u003e22.7 The Larval Fin Regeneration Model 316\u003c\/p\u003e \u003cp\u003e22.8 Summary 317\u003c\/p\u003e \u003cp\u003eAcknowledgments 317\u003c\/p\u003e \u003cp\u003eReferences 317\u003c\/p\u003e \u003cp\u003eIndex 321\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley","offers":[{"title":"Brand New","offer_id":52276257259800,"sku":"9780470425138","price":92.39,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780470425138.jpg?v=1781365836","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/zebrafish-methods-for-assessing-drug-safety-and-toxicity-hardback-9780470425138","provider":"Freshly Printed Books","version":"1.0","type":"link"}