{"product_id":"tumor-immunology-and-immunotherapy-cellular-methods-part-b-hardback-9780128186756","title":"Tumor Immunology and Immunotherapy - Cellular Methods Part B (Hardback) 9780128186756","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eTumor Immunology and Immunotherapy - Cellular Methods Part B\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cp\u003eAddressed to experts in the field who may want to expand their technical horizons and to newcomers who need detailed introductions to basic techniques\u003c\/p\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eLorenzo Galluzzi (Volume editor), Nils-Petter Rudqvist (Volume editor)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780128186756, Elsevier Science\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 28 January 2020\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e586 pages\u003cbr\u003e22.9 x 15.1 x 3.3 cm, 1 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e\u003ci\u003eTumor Immunology and Immunotherapy – Cellular Methods Part B, Volume 632\u003c\/i\u003e, the latest release in the \u003ci\u003eMethods in Enzymology\u003c\/i\u003e series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered include Quantitation of calreticulin exposure associated with immunogenic cell death, Side-by-side comparisons of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death, Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry, Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies, Methods to assess DC-dependent priming of T cell responses by dying cells, and more. \u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e1. Quantitation of calreticulin exposure associated with immunogenic cell death \u003ci\u003ePeng Liu, Liwei Zhao, Oliver Kepp and Guido Kroemer \u003c\/i\u003e2. Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death \u003ci\u003eLenka Kasikova, Iva Truxova, Isabelle Cremer, Catherine Sautes-Fridman, Oliver Kepp, Guido Kroemer, Radek Spisek and Jitka Fucikova \u003c\/i\u003e3. Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry \u003ci\u003eGiulia Cerrato, Peng Liu, Isabelle Martins, Oliver Kepp and Guido Kroemer \u003c\/i\u003e4. Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies \u003ci\u003eMartina Musella, Gwenola Manic, Claudia Galassi, Ilio Vitale and Antonella Sistigu \u003c\/i\u003e5. Methods to assess DC-dependent priming of T cell responses by dying cells \u003ci\u003eAnne-Marie Chalupova, Sarka Vosahlikova, Daniela Rozkova, Klara Sochorova, Lenka Palova-Jelinkova, Jirina Bartunkova, Radek Spisek and Jitka Fucikova \u003c\/i\u003e6. Isolation of mononuclear phagocytes from the mouse gut \u003ci\u003ePailin Chiaranunt, Kyle Burrows, Louis Ngai and Arthur Mortha \u003c\/i\u003e7. Generation of murine bone marrow-derived macrophages and using tumor coculture assays to mimic tumor microenvironment \u003ci\u003eEsra Güç, Demi Brownlie, Carolina Rodriguez Tirado, Takanori Kitamura and Jeffrey W. Pollard \u003c\/i\u003e8. Methods\u003ci\u003e \u003c\/i\u003efor macrophage differentiation and \u003ci\u003ein vitro\u003c\/i\u003e generation of human tumor associated-like macrophages \u003ci\u003eEsther Heideveld, Marta Horcas-Lopez, Martha Lopez-Yrigoyen, Lesley M. Forrester, Luca Cassetta and Jeffrey W. Pollard \u003c\/i\u003e9. In vitro assessment of cancer cell-induced polarization of macrophages \u003ci\u003eDanique E.M. Duits, Max D. Wellenstein and Karin E. de Visser \u003c\/i\u003e10. Protocol to assess the suppression of T-cell proliferation by human MDSC \u003ci\u003eAnnika M. Bruger, Christophe Vanhaver, Kirsten Bruderek, Giada Amodio, Ece Tavukçuoglu, Günes Esendagli, Silvia Gregori, Sven Brandau and Pierre van der Bruggen \u003c\/i\u003e11. Detection and functional evaluation of arginase-1 isolated from human PMNs and murine MDSC \u003ci\u003eStefania Canè and Vincenzo Bronte \u003c\/i\u003e12. Functional assay to assess T-cell inhibitory properties of myeloid derived suppressor cells (MDSCs) isolated from the tumor microenvironment of murine glioma models \u003ci\u003eMahmoud S. Alghamri, Neha Kamran, Padma Kadiyala, Pedro R. Lowenstein and Maria G. Castro \u003c\/i\u003e13. FACS isolation and analysis of human circulating and tumor neutrophils \u003ci\u003eAgnieszka Swierczak and Jeffrey W. Pollard \u003c\/i\u003e14. Flow cytometry-based isolation of tumor-associated regulatory T cells and assessment of their suppressive potential \u003ci\u003eKevin Kos, Martijn van Baalen, Denize A. Meijer and Karin E. de Visser \u003c\/i\u003e15. Assessment of lipid load in tumor-infiltrating Tregs by flow cytometry \u003ci\u003eIlenia Pacella, Alfonso Grimaldi and Silvia Piconese \u003c\/i\u003e16. Preparation of single cells from tumors for single-cell RNA sequencing \u003ci\u003eYonina Bykov, Sarah H. Kim and Dmitriy Zamarin \u003c\/i\u003e17. Characterization of the tumor immune infiltrate by multiparametric flow cytometry and unbiased high-dimensional data analysis \u003ci\u003eRomina E. Araya and Romina S. Goldszmid \u003c\/i\u003e18. Phenotyping of tumor infiltrating immune cells using mass-cytometry (CyTOF) \u003ci\u003ePauline Maby, Aurélien Corneau and Jérôme Galon \u003c\/i\u003e19. Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system \u003ci\u003eMaria Belen Garcia-Fabiani, Andrea Comba, Padma Kadiyala, Santiago Haase, Felipe Javier Núñez, David Altshuler, Pedro Ricardo Lowenstein and Maria Graciela Castro \u003c\/i\u003e20. Analysis of the immune status from peripheral whole blood with a single-tube multicolor flow cytometry assay \u003ci\u003eAnna-Jasmina Donaubauer, Ina Becker, Paul F. Rühle, Rainer Fietkau, Udo S. Gaipl and Benjamin Frey \u003c\/i\u003e21. \u003ci\u003eEx vivo \u003c\/i\u003eassessment of\u003ci\u003e in vivo \u003c\/i\u003eDC-targeted antibodies in pre-clinical models \u003ci\u003eQingrong Huang and Niroshana Anandasabapathy \u003c\/i\u003e22. Flow cytometry-based assessment of direct-targeting anti-cancer antibody immune effector functions \u003ci\u003eMichelle L. Miller and Olivera J. Finn \u003c\/i\u003e23. Enzyme-linked immunospot assay to monitor antigen-specific cellular immune responses in mouse tumor models \u003ci\u003eDonatella Negri, Paola Sestili, Martina Borghi, Maria Ciccolella and Laura Bracci \u003c\/i\u003e24. High-throughput analysis of cell-cell crosstalk in \u003ci\u003ead hoc\u003c\/i\u003e designed microfluidic chips for oncoimmunology applications \u003ci\u003eArianna Mencattini, Adele De Ninno, Jacopo Mancini, Luca Businaro, Eugenio Martinelli, Giovanna Schiavoni and Fabrizio Mattei \u003c\/i\u003e25. Generation of affinity ranged antigen-expressing tumor cell lines \u003ci\u003eAmaia Martinez-Usatorre and Pedro Romero \u003c\/i\u003e26. Recombinant fusion proteins for targeting dendritic cell subsets in therapeutic cancer vaccine \u003ci\u003eStéphanie Corgnac, Natalia K. Botelho, Alena Donda and Pedro Romero\u003c\/i\u003e\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Molecular biology [\u003ca title=\"See our other books on Molecular biology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Molecular%20biology%20%5BPSD%5D%22\"\u003ePSD\u003c\/a\u003e], Biochemistry [\u003ca title=\"See our other books on Biochemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Biochemistry%20%5BPSB%5D%22\"\u003ePSB\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Academic Press","offers":[{"title":"Default Title","offer_id":46648978604312,"sku":"9780128186756","price":116.49,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/products\/9780128186756.jpg?v=1694095831","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/tumor-immunology-and-immunotherapy-cellular-methods-part-b-hardback-9780128186756","provider":"Freshly Printed Books","version":"1.0","type":"link"}