{"product_id":"stem-cell-culture-hardback-9780123738769","title":"Stem Cell Culture (Hardback) 9780123738769","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eStem Cell Culture\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003eThis volume will gather together the breadth of current knowledge on the culture of human, and non-human, stem cells looking at both totipotent and puripotent stem cells from embryonic, fetal and adult tissue sources.\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eJennie P. Mather (Edited by)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780123738769, Elsevier Science\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 2 July 2008\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e416 pages\u003cbr\u003e23.4 x 19 x 2.7 cm, 1.01 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eThe purpose of \u003ci\u003eStem Cell Culture \u003c\/i\u003eis to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture.\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003eContributors to Volume 86\u003c\/p\u003e \u003cp\u003ePreface\u003c\/p\u003e \u003cp\u003eBiography\u003c\/p\u003e \u003cp\u003eDerivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Methods\u003c\/p\u003e \u003cp\u003eIII Discussion\u003c\/p\u003e \u003cp\u003eIV Materials\u003c\/p\u003e \u003cp\u003eAutogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder‐Free Conditions\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Methods and Materials\u003c\/p\u003e \u003cp\u003eIII Results\u003c\/p\u003e \u003cp\u003eIV Discussion and Summary\u003c\/p\u003e \u003cp\u003eMicroporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Rationale\u003c\/p\u003e \u003cp\u003eIII Methods\u003c\/p\u003e \u003cp\u003eIV Summary\u003c\/p\u003e \u003cp\u003eCulture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Rationale\u003c\/p\u003e \u003cp\u003eIII Methods\u003c\/p\u003e \u003cp\u003eIV Materials\u003c\/p\u003e \u003cp\u003eV Discussion\u003c\/p\u003e \u003cp\u003eCharacterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Amniotic Fluid Cell Composition and Stem Cells\u003c\/p\u003e \u003cp\u003eIII In Vitro Potential of Amniotic Fluid Stem Cells\u003c\/p\u003e \u003cp\u003eIV Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells\u003c\/p\u003e \u003cp\u003eV Discussion\u003c\/p\u003e \u003cp\u003eMethod to Isolate Mesenchymal‐Like Cells from Wharton's Jelly of Umbilical Cord\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Rationale\u003c\/p\u003e \u003cp\u003eIII Methods\u003c\/p\u003e \u003cp\u003eIV Materials\u003c\/p\u003e \u003cp\u003eV UCMSCs and Culture Characteristics\u003c\/p\u003e \u003cp\u003eVI Discussion\u003c\/p\u003e \u003cp\u003eIsolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs)\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Methods\u003c\/p\u003e \u003cp\u003eIII Concluding Remarks\u003c\/p\u003e \u003cp\u003eHepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Epithelial–Mesenchymal Relationship\u003c\/p\u003e \u003cp\u003eIII Stem Cells and Lineage Biology\u003c\/p\u003e \u003cp\u003eIV Liver Processing\u003c\/p\u003e \u003cp\u003eV Fractionation of Liver Cell Subpopulations\u003c\/p\u003e \u003cp\u003eVI Feeder Cells\u003c\/p\u003e \u003cp\u003eVII Extracellular Matrix\u003c\/p\u003e \u003cp\u003eVIII Culture Media\u003c\/p\u003e \u003cp\u003eIX Monolayer Cultures of Cells\u003c\/p\u003e \u003cp\u003eX Three‐Dimensional Systems\u003c\/p\u003e \u003cp\u003eAcknowledgments\u003c\/p\u003e \u003cp\u003eAppendix 1: Nomenclature, Glossary, and Abbreviations\u003c\/p\u003e \u003cp\u003eAppendix 2: Details on the Preparation of Buffers\/Reagents\u003c\/p\u003e \u003cp\u003eAppendix 3: Commercial Sources of Reagents\u003c\/p\u003e \u003cp\u003eAppendix 4: Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells. (See Below for Suppliers and the Location of the Suppliers)\u003c\/p\u003e \u003cp\u003eCulture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Rationale\u003c\/p\u003e \u003cp\u003eIII Methods\u003c\/p\u003e \u003cp\u003eIV Materials\u003c\/p\u003e \u003cp\u003eV Discussion\u003c\/p\u003e \u003cp\u003eVI Summary\u003c\/p\u003e \u003cp\u003ePrimary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Rationale\u003c\/p\u003e \u003cp\u003eIII Methods\u003c\/p\u003e \u003cp\u003eIV Materials\u003c\/p\u003e \u003cp\u003eV Discussion\u003c\/p\u003e \u003cp\u003eVI Summary\u003c\/p\u003e \u003cp\u003eIsolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Isolation of hMSCs from Trabecular Bone\u003c\/p\u003e \u003cp\u003eIII Isolation of hMSCs from Adipose Tissue\u003c\/p\u003e \u003cp\u003eIV Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs\u003c\/p\u003e \u003cp\u003eV Surface Marker Characterization of hMSCs\u003c\/p\u003e \u003cp\u003eVI hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways\u003c\/p\u003e \u003cp\u003eVII Summary\u003c\/p\u003e \u003cp\u003eCulture of Mesenchymal Stem\/Progenitor Cells in Adhesion‐Independent Conditions\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Suspension Culture of Bone Marrow‐Derived MPCs\u003c\/p\u003e \u003cp\u003eIII Analysis of Suspension Culture\u003c\/p\u003e \u003cp\u003eIV Mechanism of MPC Expansion in Suspension\u003c\/p\u003e \u003cp\u003eV Discussion\u003c\/p\u003e \u003cp\u003ePurification and Long‐Term Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Materials and Methods: Cell Analysis and Purification by Flow Cytometry\u003c\/p\u003e \u003cp\u003eIII Materials and Methods: Long‐Term Culture of Human Blood Vessel‐Associated Progenitor Cells\u003c\/p\u003e \u003cp\u003eIV Materials and Methods: Proliferation Kinetics of Human Myoendothelial Cells and Pericytes\u003c\/p\u003e \u003cp\u003eV Materials and Methods: Genotypic and Phenotypic Analyses of Long‐Term Cultured Cells\u003c\/p\u003e \u003cp\u003eVI Conclusion\u003c\/p\u003e \u003cp\u003eIsolation and Culture of Colon Cancer Stem Cells\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Identification of CSCs in Colon Cancer Tissue\u003c\/p\u003e \u003cp\u003eIII Isolation and Propagation of Colon CSCs\u003c\/p\u003e \u003cp\u003eIV Discussion\u003c\/p\u003e \u003cp\u003eIsolation and Establishment of Human Tumor Stem Cells\u003c\/p\u003e \u003cp\u003eI Introduction\u003c\/p\u003e \u003cp\u003eII Rationale\u003c\/p\u003e \u003cp\u003eIII Methods\u003c\/p\u003e \u003cp\u003eIV Results\u003c\/p\u003e \u003cp\u003eV Discussion\u003c\/p\u003e \u003cp\u003eVI Materials\u003c\/p\u003e \u003cp\u003eStem Cells from Cartilaginous and Bony Fish\u003c\/p\u003e \u003cp\u003eI Introduction to Models and Uses\u003c\/p\u003e \u003cp\u003eII Embryonal Stem Cells\u003c\/p\u003e \u003cp\u003eIII Tissue‐Specific Stem Cells\u003c\/p\u003e \u003cp\u003eIV Outlook and Future Contributions\u003c\/p\u003e \u003cp\u003eIndex\u003c\/p\u003e \u003cp\u003eVolume in Series\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Cellular biology [\u003ca title=\"See our other books on Cellular biology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Cellular%20biology%20%5Bcytology%5D%20%5BPSF%5D%22\"\u003ecytology PSF\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Academic Press","offers":[{"title":"Default Title","offer_id":46649278726424,"sku":"9780123738769","price":103.28,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/products\/9780123738769.jpg?v=1695013826","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/stem-cell-culture-hardback-9780123738769","provider":"Freshly Printed Books","version":"1.0","type":"link"}