{"product_id":"protein-and-peptide-mass-spectrometry-in-drug-discovery-hardback-9780470258170","title":"Protein and Peptide Mass Spectrometry in Drug Discovery (Hardback) 9780470258170","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eProtein and Peptide Mass Spectrometry in Drug Discovery\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cfont size=\"4\"\u003eMichael L. Gross (Edited by), M.L Gross (Author), Guodong Chen (Edited by), Birendra Pramanik (Edited by)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780470258170, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 23 December 2011\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e492 pages\u003cbr\u003e24.1 x 16.3 x 3 cm, 0.875 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e\"This book will be a valuable reference as it contains plenty of depth and substance to be of interest to experienced practitioners of mass spectrometry and related techniques, but is still accessible to pharmaceutical researchers who want to learn more about MS technologies and its applications.\"  (American Society for Mass Spectrometry, 1 July 2012)\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cb\u003eThe book that highlights mass spectrometry and its application in characterizing proteins and peptides in drug discovery\u003c\/b\u003e  \u003cp\u003eAn instrumental analytical method for quantifying the mass and characterization of various samples from small molecules to large proteins, mass spectrometry (MS) has become one of the most widely used techniques for studying proteins and peptides over the last decade. Bringing together the work of experts in academia and industry, \u003ci\u003eProtein and Peptide Mass Spectrometry in Drug\u003c\/i\u003e Discovery highlights current analytical approaches, industry practices, and modern strategies for the characterization of both peptides and proteins in drug discovery.\u003c\/p\u003e \u003cp\u003eIllustrating the critical role MS technology plays in characterizing target proteins and protein products, the methods used, ion mobility, and the use of microwave radiation to speed proteolysis, the book also covers important emerging applications for neuroproteomics and antigenic peptides. Placing an emphasis on the pharmaceutical industry, the book stresses practice and applications, presenting real-world examples covering the most recent advances in mass spectrometry, and providing an invaluable resource for pharmaceutical scientists in industry and academia, analytical and bioanalytical chemists, and researchers in protein science and proteomics.\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePREFACE xv  \u003cp\u003eCONTRIBUTORS xvii\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART I METHODOLOGY 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Ionization Methods in Protein Mass Spectrometry 3\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eIsmael Cotte-Rodriguez, Yun Zhang, Zhixin Miao, and Hao Chen\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 History of the Development of Protein Mass Spectrometry 4\u003c\/p\u003e \u003cp\u003e1.2 Laser-Based Ionization Methods for Proteins 5\u003c\/p\u003e \u003cp\u003e1.3 Spray-Based Ionization Methods for Proteins 13\u003c\/p\u003e \u003cp\u003e1.4 Ambient Ionization Methods 20\u003c\/p\u003e \u003cp\u003e1.5 Conclusions 30\u003c\/p\u003e \u003cp\u003eAcknowledgments 30\u003c\/p\u003e \u003cp\u003eReferences 30\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Ion Activation and Mass Analysis in Protein Mass Spectrometry 43\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eCheng Lin and Peter O’Connor\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 43\u003c\/p\u003e \u003cp\u003e2.2 Ion Activation and Tandem MS Analysis 46\u003c\/p\u003e \u003cp\u003e2.3 Mass Analyzers 59\u003c\/p\u003e \u003cp\u003eReferences 81\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Target Proteins: Bottom-up and Top-down Proteomics 89\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMichael Boyne and Ron Bose\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Mass Spectral Approaches to Targeted Protein Identification 89\u003c\/p\u003e \u003cp\u003e3.2 Bottom-up Proteomics 90\u003c\/p\u003e \u003cp\u003e3.3 Top-down Approaches 96\u003c\/p\u003e \u003cp\u003e3.4 Next-Generation Approaches 98\u003c\/p\u003e \u003cp\u003eReferences 99\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Quantitative Proteomics by Mass Spectrometry 101\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJacob Galan, Anton Iliuk, and W. Andy Tao\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 101\u003c\/p\u003e \u003cp\u003e4.2 In-Cell Labeling 105\u003c\/p\u003e \u003cp\u003e4.3 Quantitation via Isotopic Labeling of Proteins 107\u003c\/p\u003e \u003cp\u003e4.4 Quantitation via Isotopic Labeling on Peptides 112\u003c\/p\u003e \u003cp\u003e4.5 Label-Free Quantitation 116\u003c\/p\u003e \u003cp\u003e4.6 Conclusions 119\u003c\/p\u003e \u003cp\u003eAcknowledgment 120\u003c\/p\u003e \u003cp\u003eReferences 120\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Comparative Proteomics by Direct Tissue Analysis Using Imaging Mass Spectrometry 129\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMichelle L. Reyzer and Richard M. Caprioli\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 129\u003c\/p\u003e \u003cp\u003e5.2 Conventional Comparative Proteomics 130\u003c\/p\u003e \u003cp\u003e5.3 Comparative Proteomics Using Imaging MS 131\u003c\/p\u003e \u003cp\u003e5.4 Conclusions 136\u003c\/p\u003e \u003cp\u003eAcknowledgments 137\u003c\/p\u003e \u003cp\u003eReferences 137\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Peptide and Protein Analysis Using Ion Mobility–Mass Spectrometry 139\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJeffrey R. Enders, Michal Kliman, Sevugarajan Sundarapandian, and John A. McLean\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Ion Mobility–Mass Spectrometry: Instrumentation and Separation Selectivity 139\u003c\/p\u003e \u003cp\u003e6.2 Characterizing and Interpreting Peptide and Protein Structures 147\u003c\/p\u003e \u003cp\u003e6.3 Applications of IM-MS to Peptide and Protein Characterizations 152\u003c\/p\u003e \u003cp\u003e6.4 Future Directions 158\u003c\/p\u003e \u003cp\u003eAcknowledgments 159\u003c\/p\u003e \u003cp\u003eReferences 160\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Chemical Footprinting for Determining Protein Properties and Interactions 175\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSandra A. Kerfoot and Michael L. Gross\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction to Hydrogen–Deuterium Exchange 175\u003c\/p\u003e \u003cp\u003e7.2 Experimental Procedures 178\u003c\/p\u003e \u003cp\u003e7.3 Mass Spectrometry-Based HDX in Practice 182\u003c\/p\u003e \u003cp\u003e7.4 Protein Footprinting via Free-Radical Oxidation 193\u003c\/p\u003e \u003cp\u003e7.5 Chemical Crosslinking 198\u003c\/p\u003e \u003cp\u003e7.6 Selective and Irreversible Chemical Modification 201\u003c\/p\u003e \u003cp\u003e7.7 Conclusion 205\u003c\/p\u003e \u003cp\u003eReferences 206\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Microwave Technology to Accelerate Protein Analysis 213\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eUrooj A. Mirza, Birendra N. Pramanik, and Ajay K. Bose\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 213\u003c\/p\u003e \u003cp\u003e8.2 Microwave Technology 215\u003c\/p\u003e \u003cp\u003e8.3 Summary 224\u003c\/p\u003e \u003cp\u003eAcknowledgments 224\u003c\/p\u003e \u003cp\u003eReferences 224\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Bioinformatics and Database Searching 231\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSurendra Dasari and David L. Tabb\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Overview 231\u003c\/p\u003e \u003cp\u003e9.2 Introduction to Tandem Mass Spectrometry 231\u003c\/p\u003e \u003cp\u003e9.3 Overview of Peptide Identification with Database Searching 234\u003c\/p\u003e \u003cp\u003e9.4 MyriMatch-IDPicker Protein Identification Pipeline 235\u003c\/p\u003e \u003cp\u003e9.5 Results of a Shotgun Proteomics Study 246\u003c\/p\u003e \u003cp\u003e9.6 Improvements to MyriMatch Database Search Engine 248\u003c\/p\u003e \u003cp\u003e9.7 Applications of MyriMatch-IDPicker Pipeline 250\u003c\/p\u003e \u003cp\u003e9.8 Conclusions 251\u003c\/p\u003e \u003cp\u003eAcknowledgments 251\u003c\/p\u003e \u003cp\u003eReferences 251\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART II Applications 253\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Mass Spectrometry-Based Screening and Characterization of Protein–Ligand Complexes in Drug Discovery 255\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eChristine L. Andrews, Michael R. Ziebell, Elliott Nickbarg, and Xianshu Yang\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 255\u003c\/p\u003e \u003cp\u003e10.2 Affinity Selection Mass Spectrometry (AS-MS) 256\u003c\/p\u003e \u003cp\u003e10.3 Solution-Based AS-MS as Screening Technologies 258\u003c\/p\u003e \u003cp\u003e10.4 Gas-Phase Interactions 267\u003c\/p\u003e \u003cp\u003e10.5 Enzyme Activity Assays Using MS for Screening or Confirming Drug Candidates 271\u003c\/p\u003e \u003cp\u003e10.6 Conclusions and Future Directions 276\u003c\/p\u003e \u003cp\u003eReferences 277\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Utilization of Mass Spectrometry for the Structural Characterization of Biopharmaceutical Protein Products 287\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAmareth Lim and Catherine A. Srebalus Barnes\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 287\u003c\/p\u003e \u003cp\u003e11.2 MS-Based Approach for the Characterization of Recombinant Therapeutic Proteins 288\u003c\/p\u003e \u003cp\u003e11.3 Cell Culture Development 290\u003c\/p\u003e \u003cp\u003e11.4 Purification Development 294\u003c\/p\u003e \u003cp\u003e11.5 Formulation Development 300\u003c\/p\u003e \u003cp\u003e11.6 Analytical Method Development 304\u003c\/p\u003e \u003cp\u003e11.7 Confirmation of Structure\/Product Comparability Assessment 311\u003c\/p\u003e \u003cp\u003e11.8 Conclusions 313\u003c\/p\u003e \u003cp\u003eAcknowledgments 315\u003c\/p\u003e \u003cp\u003eReferences 315\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Post-translationally Modified Proteins: Glycosylation, Phosphorylation, and Disulfide Bond Formation 321\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAnthony Tsarbopoulos and Fotini N. Bazoti\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 321\u003c\/p\u003e \u003cp\u003e12.2 Glycosylation 322\u003c\/p\u003e \u003cp\u003e12.3 Phosphorylation 338\u003c\/p\u003e \u003cp\u003e12.4 Disulfide Bond Detection and Mapping 347\u003c\/p\u003e \u003cp\u003e12.5 Future Perspectives 350\u003c\/p\u003e \u003cp\u003eAcknowledgments 352\u003c\/p\u003e \u003cp\u003eAbbreviations 353\u003c\/p\u003e \u003cp\u003eReferences 354\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Mass Spectrometry of Antigenic Peptides 371\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eHenry Rohrs\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 371\u003c\/p\u003e \u003cp\u003e13.2 Analysis of Antigenic Peptides 374\u003c\/p\u003e \u003cp\u003e13.3 Examples of the Application of Mass Spectrometry to Antigenic Peptide Study 381\u003c\/p\u003e \u003cp\u003e13.4 Future Work 385\u003c\/p\u003e \u003cp\u003eAcknowledgments 386\u003c\/p\u003e \u003cp\u003eAbbreviations 387\u003c\/p\u003e \u003cp\u003eReferences 387\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Neuropeptidomics 393\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJonathan V. Sweedler, Fang Xie, and Adriana Bora\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction 393\u003c\/p\u003e \u003cp\u003e14.2 Neuropeptidomics: Characterizing Peptides in the Brain 394\u003c\/p\u003e \u003cp\u003e14.3 Sample Preparation for Mass Spectrometry 395\u003c\/p\u003e \u003cp\u003e14.4 Separations 405\u003c\/p\u003e \u003cp\u003e14.5 Peptide Characterization via Mass Spectrometry 407\u003c\/p\u003e \u003cp\u003e14.6 Conclusions 419\u003c\/p\u003e \u003cp\u003e14.7 Future Perspectives 419\u003c\/p\u003e \u003cp\u003eAcknowledgments 420\u003c\/p\u003e \u003cp\u003eReferences 420\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Mass Spectrometry for the Study of Peptide Drug Metabolism 435\u003cbr\u003e \u003c\/b\u003e\u003ci\u003ePatrick J. Rudewicz\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction 435\u003c\/p\u003e \u003cp\u003e15.2 Peptide Drug Metabolism 436\u003c\/p\u003e \u003cp\u003e15.3 LC-MS\/MS for Metabolite Identification 437\u003c\/p\u003e \u003cp\u003e15.4 Quantitative Analysis 439\u003c\/p\u003e \u003cp\u003e15.5 Case Study: IL-1b Protease Inhibitors 440\u003c\/p\u003e \u003cp\u003e15.6 Future Directions 445\u003c\/p\u003e \u003cp\u003eReferences 445\u003c\/p\u003e \u003cp\u003eINDEX 449\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley","offers":[{"title":"Brand New","offer_id":52257257586968,"sku":null,"price":0.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780470258170.jpg?v=1781278088","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/protein-and-peptide-mass-spectrometry-in-drug-discovery-hardback-9780470258170","provider":"Freshly Printed Books","version":"1.0","type":"link"}