{"product_id":"practical-cell-analysis-hardback-9780470741559","title":"Practical Cell Analysis (Hardback) 9780470741559","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003ePractical Cell Analysis\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cfont size=\"4\"\u003eDimitri Pappas (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780470741559, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 19 February 2010\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e314 pages\u003cbr\u003e23.6 x 15.8 x 2.3 cm, 0.576 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e\"It is a valuable means to get started in the field and targets mainly scientists without prior experience in cell biology . . . In summary, the book has earned its place on the bench in an interdisciplinary life science environment.\" (Anal Bioanal Chem, 2010)\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eAs analytical chemistry and biology move closer together, biologists are performing increasingly sophisticated analytical techniques on cells. Chemists are also turning to cells as a relevant and important sample to study newly developed methods. \u003ci\u003ePractical Cell Analysis\u003c\/i\u003e provides techniques, hints, and time-saving tips explaining what may be “common knowledge” to one field but are often hidden or unknown to another.  \u003cp\u003eWithin this practical guide:\u003c\/p\u003e \u003cul type=\"disc\"\u003e \u003cli\u003eThe procedures and protocols for cell separation, handling cells on a microscope and for using cells in microfluidic devices are presented.\u003c\/li\u003e \u003cli\u003eElements of cell culture are taken and combined with the practical advice necessary to maintain a cell lab and to handle cells properly during an analysis\u003c\/li\u003e \u003cli\u003eThe main chapters deal with the fundamentals and applied aspects of each technique, with one complete chapter focusing on statistical considerations of analyzing cells\u003c\/li\u003e \u003cli\u003eMany diagram-based protocols for some of the more common cell processes are included\u003c\/li\u003e \u003cli\u003eChapter summaries and extensive tables are included so that key information can be looked up easily in the lab setting\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eMuch like a good manual or cookbook this book is a useful, practical guide and a handy reference for all students, researchers and practitioners involved in cellular analysis.\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cb\u003eChapter 1: Getting Starting (and Getting the Cells)\u003c\/b\u003e  \u003cp\u003e1.1 Introduction\u003c\/p\u003e \u003cp\u003e1.2 The Driving Need\u003c\/p\u003e \u003cp\u003e1.3 Primary and Cultured Cells\u003c\/p\u003e \u003cp\u003e1.4 Choosing a Cultured Cell\u003c\/p\u003e \u003cp\u003e1.5 Choosing Primary Cells\u003c\/p\u003e \u003cp\u003e1.6 Easily Obtainable Primary Cells\u003c\/p\u003e \u003cp\u003e1.7 Primary Cells from Tissues\u003c\/p\u003e \u003cp\u003e1.8 Purifying Primary Cells\u003c\/p\u003e \u003cp\u003e1.9 How Long do Primary Cells Remain Primary?\u003c\/p\u003e \u003cp\u003e1.10 Obtaining Primary Cells from a Commercial Source\u003c\/p\u003e \u003cp\u003e1.11 Bacteria and Yeast\u003c\/p\u003e \u003cp\u003e1.12 Practical Aspects of Cell Culture\u003c\/p\u003e \u003cp\u003e1.13 Safety Aspects of Primary and Transformed Cell Lines\u003c\/p\u003e \u003cp\u003e1.14 Transfection of Primary and Transformed Cell Lines\u003c\/p\u003e \u003cp\u003e1.15 Conclusion\u003c\/p\u003e \u003cp\u003eReferences\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 2: The Cell Culture Laboratory (Tools of the Trade)\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction\u003c\/p\u003e \u003cp\u003e2.2 Issues Concerning a Cell Laboratory\u003c\/p\u003e \u003cp\u003e2.3 Setting up a Cell Culture Laboratory\u003c\/p\u003e \u003cp\u003e2.4 Cell Line Storage\u003c\/p\u003e \u003cp\u003e2.5 Personal Protective Equipment\u003c\/p\u003e \u003cp\u003e2.6 Cell and Sample Handling\u003c\/p\u003e \u003cp\u003e2.7 Common Analytical Instrumentation for Cell Culture\u003c\/p\u003e \u003cp\u003e2.8 Considerations when Setting up a Cell Culture Laboratory\u003c\/p\u003e \u003cp\u003e2.9 Establishing and Regulating A Culture Facility\u003c\/p\u003e \u003cp\u003e2.10 Conclusion\u003c\/p\u003e \u003cp\u003eReferences\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 3. Maintaining Cultures\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction\u003c\/p\u003e \u003cp\u003e3.2 Medium\u003c\/p\u003e \u003cp\u003e3.3 The Use of Medium in Analysis, and Alternatives\u003c\/p\u003e \u003cp\u003e3.4 Culturing Cells\u003c\/p\u003e \u003cp\u003eProtocol 3.1: Sub-Culture of Adherent Cells\u003c\/p\u003e \u003cp\u003e3.5 Growing Cells in Three Dimensions\u003c\/p\u003e \u003cp\u003e3.6 Sterility and Contamination of Culture\u003c\/p\u003e \u003cp\u003e3.7 Storage of Cell Samples and Cell Lines\u003c\/p\u003e \u003cp\u003eProtocol 3.2: Cryopreservation of Mammalian Cells\u003c\/p\u003e \u003cp\u003eProtocol 3.3: Retrieval of Cells from Liquid Nitrogen Storage\u003c\/p\u003e \u003cp\u003e3.8 Conclusion\u003c\/p\u003e \u003cp\u003eReferences\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 4. Microscopy of Cells\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction\u003c\/p\u003e \u003cp\u003e4.2 Microscope Types\u003c\/p\u003e \u003cp\u003e4.3 Culturing Cells for Microscopy\u003c\/p\u003e \u003cp\u003e4.4 Signals, Background, and Artifacts in Optical Microscopy\u003c\/p\u003e \u003cp\u003e4.5 Staining Cells for Fluorescence Microscopy\u003c\/p\u003e \u003cp\u003e4.6 Multiple Labels \u003c\/p\u003e \u003cp\u003e4.7 Viability and Two-Photon Microscopy\u003c\/p\u003e \u003cp\u003e4.8 Spatial Resolution in Optical Microscopy\u003c\/p\u003e \u003cp\u003e4.9 Image Saturation and Intensity\u003c\/p\u003e \u003cp\u003e4.10 Atomic Force and Environmental Scanning Electron Microscopy\u003c\/p\u003e \u003cp\u003e4.11 Conclusion\u003c\/p\u003e \u003cp\u003eReferences\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 5: Separating Cells\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction\u003c\/p\u003e \u003cp\u003e5.2 The Cell Sample\u003c\/p\u003e \u003cp\u003e5.3 Label-Free (Intrinsic) Separations\u003c\/p\u003e \u003cp\u003e5.4 Immunomagnetic Sorting\u003c\/p\u003e \u003cp\u003e5.5 Cell Affinity Chromatography\u003c\/p\u003e \u003cp\u003e5.6 Affinity Chemistry Considerations in CAC and MACS Separations\u003c\/p\u003e \u003cp\u003eProtocol 5.1: Screening of Antibody Clones\u003c\/p\u003e \u003cp\u003e5.7 Elution in Cell Affinity Chromatography\u003c\/p\u003e \u003cp\u003e5.8 Nonspecific Binding in Cell Separations\u003c\/p\u003e \u003cp\u003e5.9 Separation of Rare Cells\u003c\/p\u003e \u003cp\u003e5.10 Fluorescence Activate Cell Sorting\u003c\/p\u003e \u003cp\u003e5.11 Sorting Parameters\u003c\/p\u003e \u003cp\u003e5.12 Other Separation Techniques and Considerations\u003c\/p\u003e \u003cp\u003e5.13 Conclusion\u003c\/p\u003e \u003cp\u003eReferences\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 6. Flow Cytometry: Cell Analysis in the Fast Lane\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction\u003c\/p\u003e \u003cp\u003e6.2 The Cell Sample\u003c\/p\u003e \u003cp\u003e6.3 Flow Cytometer Function\u003c\/p\u003e \u003cp\u003e6.4 Obtaining or Finding a Flow Cytometer\u003c\/p\u003e \u003cp\u003e6.5 Using Flow Cytometers\u003c\/p\u003e \u003cp\u003e6.6 Setting up a Flow Cytometer for Multi-Color Staining\u003c\/p\u003e \u003cp\u003e6.7 Analyzing Flow Cytometry Data\u003c\/p\u003e \u003cp\u003e6.8 Example Flow Cytometry Assays\u003c\/p\u003e \u003cp\u003e6.9 No-Flow Cytometry \u003c\/p\u003e \u003cp\u003e6.10 Conclusion\u003c\/p\u003e \u003cp\u003eReferences\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 7: Analyzing Cells with Microfluidic Devices\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction\u003c\/p\u003e \u003cp\u003e7.2 Advantages of Microfluidics\u003c\/p\u003e \u003cp\u003e7.3 Considerations of Microfluidics and Cells\u003c\/p\u003e \u003cp\u003e7.4 Obtaining Microfluidic Cell Devices\u003c\/p\u003e \u003cp\u003e7.5 Microfluidic Flow Cytometry\u003c\/p\u003e \u003cp\u003e7.6 Cell Separations\u003c\/p\u003e \u003cp\u003e7.7 Analysis of Cell Products\u003c\/p\u003e \u003cp\u003e7.8 Cell Culture\u003c\/p\u003e \u003cp\u003eProtocol 7.1: Low-shear Cell Culture Chip\u003c\/p\u003e \u003cp\u003e7.9 Conclusion\u003c\/p\u003e \u003cp\u003eReferences\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 8: Statistical Considerations\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction\u003c\/p\u003e \u003cp\u003e8.2 Types of Error\u003c\/p\u003e \u003cp\u003e8.3 Figures of Merit in Statistical Analysis of Cells\u003c\/p\u003e \u003cp\u003e8.4 Limits of Detection and Quantitation (of Cell)\u003c\/p\u003e \u003cp\u003e8.5 Methods to Improve Cell Statistics\u003c\/p\u003e \u003cp\u003e8.6 Comparing Analytical Values\u003c\/p\u003e \u003cp\u003e8.7 Rejecting Data: Proceed With Caution\u003c\/p\u003e \u003cp\u003e8.8 Conclusion\u003c\/p\u003e \u003cp\u003eReferences\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 9 Protocols, Probes, and Standards\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction\u003c\/p\u003e \u003cp\u003e9.2 Cell Transfection and Immortalization (Chapter 1)\u003c\/p\u003e \u003cp\u003eProtocol 9.1: Transfecting Cells with Polyamine Reagents\u003c\/p\u003e \u003cp\u003eProtocol 9.2: Stable Transfection using Polyamine Delivery\u003c\/p\u003e \u003cp\u003eProtocol 9.3: Transfection Using Electroporation\u003c\/p\u003e \u003cp\u003eOptimizing Electroporation Parameters\u003c\/p\u003e \u003cp\u003eProtocol 9.4: Cell Immortalization using hTERT Transfection\u003c\/p\u003e \u003cp\u003e9.3 Calculating Relative Centrifugal Force (RCF) and Centrifuge Rotor Speed (Chapter 2)\u003c\/p\u003e \u003cp\u003e9.4 Fluorescence Methods (Chapter 4 \u0026amp; 6)\u003c\/p\u003e \u003cp\u003eProtocol 9.4: Apoptosis Detection Using Fluorophore-Conjugated Annexin-V and a Viability Dye\u003c\/p\u003e \u003cp\u003eProtocol 9.5: Apoptosis Detection Using Fluorogenic Caspase Probes\u003c\/p\u003e \u003cp\u003e9.5 Surface Modifications for Cell Analysis (Chapter 5 \u0026amp; 7)\u003c\/p\u003e \u003cp\u003eProtocol 9.6: Covalent Linkage of Proteins (non-Antibody) to Glass by Microcontact Imprinting\u003c\/p\u003e \u003cp\u003eProtocol 9.7: Covalent Linkage of Antibodies to Glass\u003c\/p\u003e \u003cp\u003eProtocol 9.8: Noncovalent Attachment of Antibodies to Glass #1\u003c\/p\u003e \u003cp\u003eProtocol 9.9: Noncovalent Attachment of Antibodies to Glass or PDMS #2\u003c\/p\u003e \u003cp\u003eProtocol 9.10: Blocking Endogenous Biotin\u003c\/p\u003e \u003cp\u003e9.6 Flow Cytometry and Cell Separations (Chapter 5 \u0026amp; 6)\u003c\/p\u003e \u003cp\u003eProtocol 9.11: Cell Cycle Measurements by Flow Cytometry\u003c\/p\u003e \u003cp\u003eProtocol 9.12: Antigen Density Measurements in Flow Cytometry\u003c\/p\u003e \u003cp\u003eProtocol 9.13: Antigen Density Measurements using Fluorescence Correlation Spectroscopy.\u003c\/p\u003e \u003cp\u003eProtocol 9.14: Cell Proliferation using Anti-CD71 staining (Chapter 4 \u0026amp; 6).\u003c\/p\u003e \u003cp\u003e9.7 Fluorescent Labels and Fluorogenic Probes (Chapter 4, 6 \u0026amp; 7)\u003c\/p\u003e \u003cp\u003eReferences\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley","offers":[{"title":"Brand New","offer_id":52278025945368,"sku":"9780470741559","price":66.39,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780470741559.jpg?v=1781456400","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/practical-cell-analysis-hardback-9780470741559","provider":"Freshly Printed Books","version":"1.0","type":"link"}