{"product_id":"pcr-guru-an-ultimate-benchtop-reference-for-molecular-biologists-paperback-9780128042311","title":"PCR Guru; An Ultimate Benchtop Reference for Molecular Biologists (Paperback \/ softback) 9780128042311","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003ePCR Guru\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eAn Ultimate Benchtop Reference for Molecular Biologists\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cem\u003e\u003cp\u003eProvides researchers in molecular biology with a handy reference to help solve challenging problems in PCR setup and optimization\u003c\/p\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eAyaz Najafov (Author), Gerta Hoxhaj (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780128042311\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePaperback \/ softback, published 9 December 2016\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e104 pages\u003cbr\u003e22.9 x 15.1 x 0.8 cm, 0.2 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e\u003ci\u003ePCR Guru: An Ultimate Benchtop Reference for Molecular Biologists\u003c\/i\u003e is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform PCR.\u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e1. Introduction2. Procedure3. Good PCR Practices4. Troubleshooting5. Tips6. Special CasesAppendix A. Agarose Gel Electrophoresis ProtocolAppendix B. Silver Staining of DNA GelsAppendix C. Plasmid Miniprep ProtocolAppendix D. Restriction Enzyme Digestion ProtocolAppendix E. Protocol for DNA Concentration Estimation by using Hoechst 33258Appendix F. FormulasAppendix G. Primers for Common Promoters, Housekeeping Genes, Reporters, etc.Appendix H. TagsAppendix I. Thermostable DNA polymerasesAppendix J. Example of a Daily PCR LogAppendix K. Common Buffers and SolutionsAppendix L. Useful numbersAppendix M. Yeast colony PCR\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Genetics [\u003ca title=\"See our other books on Genetics\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Genetics%20%5Bnon-medical%5D%20%5BPSAK%5D%22\"\u003enon-medical PSAK\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Freshly Printed Books","offers":[{"title":"Default Title","offer_id":46649010913560,"sku":"9780128042311","price":46.99,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/products\/9780128042311_b1e9987e-d6a3-497f-9dea-254694f1a9d6.jpg?v=1694353146","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/pcr-guru-an-ultimate-benchtop-reference-for-molecular-biologists-paperback-9780128042311","provider":"Freshly Printed Books","version":"1.0","type":"link"}