{"product_id":"mitochondria-biology-hardback-9780128202289","title":"Mitochondria Biology (Hardback) 9780128202289","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eMitochondria Biology\u003c\/font\u003e\u003cbr\u003e\r\n\r\n\r\n\u003c\/p\u003e\n\u003cp\u003e\u003cem\u003e\u003cp\u003ePresents the tried-and-tested methods to study mitochondria for both new and veteran mitochondriacs\u003c\/p\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eLiza A. Pon (Volume editor), Eric A. Schon (Volume editor)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780128202289, Elsevier Science\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 13 March 2020\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e604 pages\u003cbr\u003e23.4 x 19 x 3.4 cm, 1.38 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003e\u003cp\u003e\u003ci\u003eMethods in Cell Biology Volume 155 \u003c\/i\u003eprovides an update on the step-by-step \"how-to\" methods to study mitochondrial structure, function and biogenesis contained in the first two editions. As in the previous editions, biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls for each method. New chapters in this update include Isolation of Mitochondria and Analysis of Mitochondrial Compartments, Isolation of Mitochondria from Animal Cells and Yeast, Isolation and Characterization of Mitochondria-Associated ER Membranes, Import of Proteins into Mitochondria, Proximity Labeling Methods to Assess Protein-Protein Interactions in Yeast Mitochondria, and more. \u003c\/p\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cb\u003ePart 1 - Isolation of Mitochondria and Analysis of Mitochondrial Compartments \u003c\/b\u003e1. Isolation of mitochondria from animal cells and yeast \u003ci\u003eFrancesco Pallotti and Liza Pon \u003c\/i\u003e2. Isolation and characterization of mitochondria-associated ER membranes \u003ci\u003eEstela Area-Gomez \u003c\/i\u003e3. Import of proteins into mitochondria \u003ci\u003eNikolaus Pfanner and Thomas Becker \u003c\/i\u003e4. Proximity labeling methods to assess protein-protein interactions in yeast mitochondria \u003ci\u003eJie-Ning Yang and Liza Pon\u003c\/i\u003e  \u003cp\u003e\u003cb\u003ePart 2 - Assays for Mitochondrial Metabolic Activities \u003c\/b\u003e5. Biochemical assays of TCA cycle and ß-oxidation metabolites \u003ci\u003eMichael J. Bennett, Sheng Feng and Ann Saada (Reisch) \u003c\/i\u003e6. Biochemical assays of respiratory chain complex activity  \u003ci\u003eRobert Taylor \u003c\/i\u003e7. Assessing mitochondrial bioenergetics in whole cells and isolated organelles by microplate respirometry and polarography \u003ci\u003eErich Gnaiger and Orian Shirihai \u003c\/i\u003e8. Analyzing electron transport chain supercomplexes \u003ci\u003eJose Antonio Enriquez \u003c\/i\u003e9. Assays of mitochondrial ATP synthesis in animal cells and tissues \u003ci\u003ePaolo Pinton and Hiromi Imamura \u003c\/i\u003e10. Visualization of mitochondrial membrane potential in mammalian cells \u003ci\u003eNoemi Esteras, Merel Adjobo-Hermans, Andray Abramov and Werner JH Koopman \u003c\/i\u003e11. Visualization of mitochondrial function in living cells using biosensors (SoNAR, ro-GFP, DiOC6\/Tom70 mCherry; DHE; Mitosox) \u003ci\u003eYi Yang and Liza Pon\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 3 - Assays for Other Mitochondrial Activities \u003c\/b\u003e12. Histochemical methods to measure respiratory chain activity and visualize mitochondria and mitochondrial gene products  \u003ci\u003eKurenai Tanji and Sandra Franco-Iborra \u003c\/i\u003e13. Analysis of mitochondrial phospholipids and cardiolipin \u003ci\u003eMichael Schlame and Estela Area-Gomez \u003c\/i\u003e14. Biosensors for Detection of Calcium and pH \u003ci\u003eDiego Destefani \u003c\/i\u003e15. Measurement of membrane permeability and the mitochondrial permeability transition  \u003ci\u003ePaolo Bernardi and Michela Carraro\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 4 - Mitochondrial Genes and Gene Expression  \u003c\/b\u003e16. Detection of mtDNA mutations \u003ci\u003eAli Naini, Sara Shanske, Robert Gilkerson and Jiuhong Pang \u003c\/i\u003e17. Visualization of mitochondrial DNA replication \u003ci\u003eAgnel Sfeir \u003c\/i\u003e18. Cybrid technology  \u003ci\u003eCarlos Moraes and Sandra Bacman \u003c\/i\u003e19. Manipulation of mitochondrial genes and mtDNA heteroplasmy \u003ci\u003eCarlos Moraes, Sandra Bacman and Michal Minczuk\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 5 - Assays for Mitochondrial Dynamics \u003c\/b\u003e20. Assays for mitochondrial fusion and fission  \u003ci\u003eLaura Lackner, Jason Lee, Jason Mears and Suzanne Hoppins \u003c\/i\u003e21. Methods to visualize mitochondria and mitochondrial motility \u003ci\u003eLiza Pon, Theresa Swayne, Ryo Higuchi-Sanabria and Pin-Chao Liao \u003c\/i\u003e22. In vitro and in vivo detection of mitophagy in animal cells, mice, and yeast (Keima; red\/green mitophagosomes)\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendices \u003c\/b\u003eA. Basic properties of mitochondria \u003ci\u003eLuis García-Rodríguez \u003c\/i\u003eB. Inhibitors of the respiratory chain and of ATP synthesis \u003ci\u003eNanette Orme-Johnson and Eric A. Schon \u003c\/i\u003eC. Linearized maps of selected mitochondrial genomes \u003ci\u003eEric Schon \u003c\/i\u003eD. Mitochondrial genetic codes in various organisms  \u003ci\u003eEric Schon \u003c\/i\u003eE. Gene products present in mitochondria of yeast and animal cells  \u003ci\u003eEric Schon\u003c\/i\u003e\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Cellular biology [\u003ca title=\"See our other books on Cellular biology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Cellular%20biology%20%5Bcytology%5D%20%5BPSF%5D%22\"\u003ecytology PSF\u003c\/a\u003e], Molecular biology [\u003ca title=\"See our other books on Molecular biology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Molecular%20biology%20%5BPSD%5D%22\"\u003ePSD\u003c\/a\u003e], Developmental biology [\u003ca title=\"See our other books on Developmental biology\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Developmental%20biology%20%5BPSC%5D%22\"\u003ePSC\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Academic Press","offers":[{"title":"Default Title","offer_id":46648698798360,"sku":"9780128202289","price":130.79,"currency_code":"GBP","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/products\/9780128202289.jpg?v=1694093415","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/mitochondria-biology-hardback-9780128202289","provider":"Freshly Printed Books","version":"1.0","type":"link"}