{"product_id":"methods-in-molecular-biology-and-protein-chemistry-cloning-and-characterization-of-an-enterotoxin-subunit-paperback-softback-9780470843604","title":"Methods in Molecular Biology and Protein Chemistry; Cloning and Characterization of an Enterotoxin Subunit (Paperback \/ softback) 9780470843604","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eMethods in Molecular Biology and Protein Chemistry\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eCloning and Characterization of an Enterotoxin Subunit\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eBrenda D. Spangler (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780470843604, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePaperback \/ softback, published 24 April 2002\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e224 pages\u003cbr\u003e28 x 21.8 x 1.4 cm, 0.539 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eEin praxisorientierter Leitfaden für Studenten im Hauptstudium.\u003cbr\u003e \u003cbr\u003e Vermittelt werden aktuelle Techniken der Molekularbiologie und Biochemie als fester Bestandteil eines Forschungsprojektes.\u003cbr\u003e \u003cbr\u003e Auf diese Weise kann der Lernende die Auswirkung der Gentechnik anhand der praktischen Anwendung von nassanalytischen biochemischen Verfahren erkennen.\u003cbr\u003e \u003cbr\u003e Mit Computer-Tutorials für Sequenzierung, Genomik und Molekülmodellierung.\u003cbr\u003e \u003cbr\u003e Mit Tipps und Hinweisen zur Sichtung der Primärliteratur, zu mündlichen Berichten, zur Protokollierung des Labortagebuchs und zum Verfassen von Berichten.\u003cbr\u003e \u003cbr\u003e Mit einer Liste von amerikanischen und europäischen kommerziellen Anbietern von Reagenzien.\u003cbr\u003e \u003cbr\u003e Verständlich und anschaulich geschrieben.\u003cbr\u003e \u003cbr\u003e Ein unverzichtbares Lehrbuch für fortgeschrittene Studenten und Doktoranden.\u003cbr\u003e \u003cbr\u003e \"Methods in Molecular Biology\" ist jedoch NICHT geeignet für aktive Forscher auf der Suche nach den neuesten hochdifferenzierten Verfahren.\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003ePreface\u003cbr\u003e \u003cbr\u003e Introduction and Background\u003cbr\u003e \u003cbr\u003e Part 1 Molecular Biology\u003cbr\u003e \u003cbr\u003e Experiment 1 Instructions for Pipetman Use\u003cbr\u003e \u003cbr\u003e Experiment 2 Preparation of Plasmid Template DNA\u003cbr\u003e \u003cbr\u003e Experiment 3 Estimation of Plasmid DNA Concentration\u003cbr\u003e PCR Amplification of LTB Gene from Plasmid DNA\u003cbr\u003e \u003cbr\u003e Experiment 4 Agarose Gel Eletrophoresis\u003cbr\u003e Recovery of PCR Product\u003cbr\u003e \u003cbr\u003e Experiment 5 Restriction Digest of LTB Insert\u003cbr\u003e Plate Preparation\u003cbr\u003e \u003cbr\u003e Experiment 6 Purification of Digested Insert and Vector by Agarose Gel Eletrophoresis\u003cbr\u003e Recovery of Digested Insert and Vector\u003cbr\u003e \u003cbr\u003e Experiment 7 Determination of DNA Concentrations\u003cbr\u003e Ligation of Insert into Vector\u003cbr\u003e \u003cbr\u003e Experiment 8 Transformation of Host Cells by Construct\u003cbr\u003e \u003cbr\u003e Experiment 9 Colony-pick PCR to Check for Presence of Insert\u003cbr\u003e Streak Plates with Potential Constructs\u003cbr\u003e Inoculate Cultures\/Run PCR\u003cbr\u003e \u003cbr\u003e Experiment 10 Agarose Gel Electrophoresis to Verify Presence of Insert\u003cbr\u003e Purification of the Plasmid Construct using a Plasmid Miniprep Kit\u003cbr\u003e \u003cbr\u003e Experiment 11 DNA Concentration Determination\u003cbr\u003e Ethanol Precipitation of Plasmid DNA\u003cbr\u003e Sequencing Reaction\u003cbr\u003e \u003cbr\u003e Experiment 12 Purification of Extension Reaction Products\u003cbr\u003e Sequencing Gel Demonstration\u003cbr\u003e \u003cbr\u003e Experiment 13 Analysis of Sequencing Data\u003cbr\u003e Verification of Insert Sequence\u003cbr\u003e \u003cbr\u003e Experiment 14 Gene Expression\u003cbr\u003e Transformation into Expression Host\u003cbr\u003e \u003cbr\u003e Experiment 15 Induction of LTB Gene Expression\u003cbr\u003e \u003cbr\u003e Part 2 Protein Chemistry\u003cbr\u003e \u003cbr\u003e Experiment 16 SDS-PAGE of Induction Time Course\u003cbr\u003e Transfer of Protein to Membrane for Western Blot\u003cbr\u003e \u003cbr\u003e Experiment 17 Visualization of Western Blot\u003cbr\u003e \u003cbr\u003e Experiment 18 Large Scale Culture Preparation\u003cbr\u003e \u003cbr\u003e Experiment 19 Isolation of LTB by Affinity Chromatography\u003cbr\u003e \u003cbr\u003e Experiment 20 SDS-PAGE of Column Fractions\u003cbr\u003e Immunoblot to verify Presence of LTB\u003cbr\u003e \u003cbr\u003e Experiment 21 Protein Concentration\u003cbr\u003e Protein Crystallization\u003cbr\u003e \u003cbr\u003e Experiment 22 Setting up an ELISA Microtiter Plate\u003cbr\u003e \u003cbr\u003e Experiment 23 ELISA of a Panel of Ganglioside Ligands\u003cbr\u003e \u003cbr\u003e Experiment 24 Protein Structure Determination: X-ray Diffraction Techniques\u003cbr\u003e \u003cbr\u003e Experiment 25 Characterization of a Protein Crystal\u003cbr\u003e \u003cbr\u003e Experiment 26 Primer Design\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Biology, life sciences [\u003ca title=\"See our other books on Biology, life sciences\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Biology,%20life%20sciences%20%5BPS%5D%22\"\u003ePS\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley","offers":[{"title":"Brand New","offer_id":52278031089944,"sku":"9780470843604","price":93.19,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780470843604.jpg?v=1781456747","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/methods-in-molecular-biology-and-protein-chemistry-cloning-and-characterization-of-an-enterotoxin-subunit-paperback-softback-9780470843604","provider":"Freshly Printed Books","version":"1.0","type":"link"}