{"product_id":"metabolome-analysis-an-introduction-hardback-9780471743446","title":"Metabolome Analysis; An Introduction (Hardback) 9780471743446","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eMetabolome Analysis\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eAn Introduction\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eSilas G. Villas-Boas (Author), Jens Nielsen (Author), Jorn Smedsgaard (Author), Michael A. E. Hansen (Author), Ute Roessner-Tunali (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780471743446, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 6 March 2007\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e336 pages\u003cbr\u003e24 x 16.6 x 2.2 cm, 0.607 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e\"Overall, this book provides a great introduction to metabolomics and at a retail price of approximately $75, it has great value. The intended audience for this book includes new metabolomics practitioners and undergraduate\/graduate students.\" (J Am Soc Mass Spectrom, 2007)  \u003cp\u003e \u003c\/p\u003e\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eProviding information on the main approaches for the analysis of metabolites, this textbook:  \u003cul\u003e \u003cli\u003eCovers basic methodologies in sample preparation and separation techniques, as well as the most recent techniques of mass spectrometry.\u003c\/li\u003e \u003cli\u003eDifferentiates between primary and secondary metabolites.\u003c\/li\u003e \u003cli\u003eIncludes four chapters discussing successful metabolome studies of different organisms.\u003c\/li\u003e \u003cli\u003eHighlights the analytical challenges of studying metabolites.\u003c\/li\u003e \u003cli\u003eIllustrates applications of metabolome analysis through the use of case studies.\u003c\/li\u003e \u003c\/ul\u003e\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003ePreface xiii\u003c\/p\u003e \u003cp\u003eList of Contributors xv\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I: Concepts and Methodology\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Metabolomics in Functional Genomics and Systems Biology 3\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 From genomic sequencing to functional genomics 3\u003c\/p\u003e \u003cp\u003e1.2 Systems biology and metabolic models 6\u003c\/p\u003e \u003cp\u003e1.3 Metabolomics 8\u003c\/p\u003e \u003cp\u003e1.4 Future perspectives 11\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 The Chemical Challenge of the Metabolome 15\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Metabolites and metabolism 15\u003c\/p\u003e \u003cp\u003e2.2 The structural diversity of metabolites 18\u003c\/p\u003e \u003cp\u003e2.2.1 The chemical and physical properties 18\u003c\/p\u003e \u003cp\u003e2.2.2 Metabolite abundance 23\u003c\/p\u003e \u003cp\u003e2.2.3 Primary and secondary metabolism 24\u003c\/p\u003e \u003cp\u003e2.3 The number of metabolites in a biological system 25\u003c\/p\u003e \u003cp\u003e2.4 Controlling rates and levels 26\u003c\/p\u003e \u003cp\u003e2.4.1 Control by substrate level 27\u003c\/p\u003e \u003cp\u003e2.4.2 Feedback and feedforward control 27\u003c\/p\u003e \u003cp\u003e2.4.3 Control by “pathway independent” regulatory molecules 27\u003c\/p\u003e \u003cp\u003e2.4.4 Allosteric control 28\u003c\/p\u003e \u003cp\u003e2.4.5 Control by compartmentalization 30\u003c\/p\u003e \u003cp\u003e2.4.6 The dynamics of the metabolism—the mass flow 31\u003c\/p\u003e \u003cp\u003e2.4.7 Control by hormones 33\u003c\/p\u003e \u003cp\u003e2.5 Metabolic channeling or metabolons 33\u003c\/p\u003e \u003cp\u003e2.6 Metabolites are arranged in networks that are part of a cellular interactome 35\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Sampling and Sample Preparation 39\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 39\u003c\/p\u003e \u003cp\u003e3.2 Quenching—the first step 41\u003c\/p\u003e \u003cp\u003e3.2.1 Overview on metabolite turnover 41\u003c\/p\u003e \u003cp\u003e3.2.2 Different methods for quenching 44\u003c\/p\u003e \u003cp\u003e3.2.3 Quenching microbial and cell cultures 44\u003c\/p\u003e \u003cp\u003e3.2.4 Quenching plant and animal tissues 50\u003c\/p\u003e \u003cp\u003e3.3 Obtaining metabolites from biological samples 52\u003c\/p\u003e \u003cp\u003e3.3.1 Release of intracellular metabolites 52\u003c\/p\u003e \u003cp\u003e3.3.2 Structure of the cell envelopes—the main barrier to be broken 52\u003c\/p\u003e \u003cp\u003e3.3.3 Cell disruption methods 58\u003c\/p\u003e \u003cp\u003e3.3.4 Nonmechanical disruption of cell envelopes 59\u003c\/p\u003e \u003cp\u003e3.3.5 Mechanical disruption of cell envelopes 66\u003c\/p\u003e \u003cp\u003e3.4 Metabolites in the extracellular medium 71\u003c\/p\u003e \u003cp\u003e3.4.1 Metabolites in solution 72\u003c\/p\u003e \u003cp\u003e3.4.2 Metabolites in the gas phase 75\u003c\/p\u003e \u003cp\u003e3.5 Improving detection via sample concentration 76\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Analytical Tools 83\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 83\u003c\/p\u003e \u003cp\u003e4.2 Choosing a methodology 84\u003c\/p\u003e \u003cp\u003e4.3 Starting point—samples 86\u003c\/p\u003e \u003cp\u003e4.4 Principles of chromatography 87\u003c\/p\u003e \u003cp\u003e4.4.1 Basics of chromatography 87\u003c\/p\u003e \u003cp\u003e4.4.2 The chromatogram and terms in chromatography 90\u003c\/p\u003e \u003cp\u003e4.5 Chromatographic systems 93\u003c\/p\u003e \u003cp\u003e4.5.1 Gas chromatography 94\u003c\/p\u003e \u003cp\u003e4.5.2 HPLC systems 102\u003c\/p\u003e \u003cp\u003e4.6 Mass spectrometry 106\u003c\/p\u003e \u003cp\u003e4.6.1 The mass spectrometer—an overview 107\u003c\/p\u003e \u003cp\u003e4.6.2 GC-MS—the EI ion source 109\u003c\/p\u003e \u003cp\u003e4.6.3 LC-MS—the ESI ion source 111\u003c\/p\u003e \u003cp\u003e4.6.4 Mass analyzer—the quadrupole 115\u003c\/p\u003e \u003cp\u003e4.6.5 Mass analyzer—the ion-trap 117\u003c\/p\u003e \u003cp\u003e4.6.6 Mass analyzer—the time-of-flight 119\u003c\/p\u003e \u003cp\u003e4.6.7 Detection and computing in MS 121\u003c\/p\u003e \u003cp\u003e4.7 The analytical work-flow 125\u003c\/p\u003e \u003cp\u003e4.7.1 Separation by chromatography 125\u003c\/p\u003e \u003cp\u003e4.7.2 Mass spectrometry 128\u003c\/p\u003e \u003cp\u003e4.7.3 General analytical considerations 129\u003c\/p\u003e \u003cp\u003e4.8 Data evaluation 129\u003c\/p\u003e \u003cp\u003e4.8.1 Structure of data 129\u003c\/p\u003e \u003cp\u003e4.8.2 The chromatographic separation 132\u003c\/p\u003e \u003cp\u003e4.8.3 Mass spectral data 133\u003c\/p\u003e \u003cp\u003e4.8.4 Exporting data for processing 135\u003c\/p\u003e \u003cp\u003e4.9 Beyond the core methods 136\u003c\/p\u003e \u003cp\u003e4.9.1 Developments in chromatography 137\u003c\/p\u003e \u003cp\u003e4.9.2 Capillary electrophoresis 139\u003c\/p\u003e \u003cp\u003e4.9.3 Tandem MS and advanced scanning techniques 141\u003c\/p\u003e \u003cp\u003e4.9.4 NMR spectrometry 143\u003c\/p\u003e \u003cp\u003e4.10 Further reading 144\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Data Analysis 146\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Organizing the data 146\u003c\/p\u003e \u003cp\u003e5.2 Scales of measurement 147\u003c\/p\u003e \u003cp\u003e5.2.1 Qualitative data 148\u003c\/p\u003e \u003cp\u003e5.2.2 Quantitative data 148\u003c\/p\u003e \u003cp\u003e5.3 Data structures 148\u003c\/p\u003e \u003cp\u003e5.4 Preprocessing of data 150\u003c\/p\u003e \u003cp\u003e5.4.1 Calibration of data 150\u003c\/p\u003e \u003cp\u003e5.4.2 Combining profile scans 151\u003c\/p\u003e \u003cp\u003e5.4.3 Filtering 152\u003c\/p\u003e \u003cp\u003e5.4.4 Centroid calculation 156\u003c\/p\u003e \u003cp\u003e5.4.5 Internal mass scale correction 156\u003c\/p\u003e \u003cp\u003e5.4.6 Binning 157\u003c\/p\u003e \u003cp\u003e5.4.7 Baseline correction 157\u003c\/p\u003e \u003cp\u003e5.4.8 Chromatographic profile matching 163\u003c\/p\u003e \u003cp\u003e5.5 Deconvolution of spectroscopic data 166\u003c\/p\u003e \u003cp\u003e5.6 Data standardization (normalization) 167\u003c\/p\u003e \u003cp\u003e5.7 Data transformations 168\u003c\/p\u003e \u003cp\u003e5.7.1 Principal component analysis 168\u003c\/p\u003e \u003cp\u003e5.7.2 Fisher discriminant analysis 171\u003c\/p\u003e \u003cp\u003e5.8 Similarities and distances between data 173\u003c\/p\u003e \u003cp\u003e5.8.1 Continuous functions 173\u003c\/p\u003e \u003cp\u003e5.8.2 Binary functions 176\u003c\/p\u003e \u003cp\u003e5.9 Clustering techniques 178\u003c\/p\u003e \u003cp\u003e5.9.1 Hierarchical clustering 178\u003c\/p\u003e \u003cp\u003e5.9.2 k-means clustering 181\u003c\/p\u003e \u003cp\u003e5.10 Classification techniques 182\u003c\/p\u003e \u003cp\u003e5.10.1 Decision theory 183\u003c\/p\u003e \u003cp\u003e5.10.2 k-nearest neighbor 184\u003c\/p\u003e \u003cp\u003e5.10.3 Tree-based classification 184\u003c\/p\u003e \u003cp\u003e5.11 Integrated tools for automation libraries and data evaluation 185\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II—case Studies and Reviews\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Yeast Metabolomics: The Discovery of New Metabolic Pathways in Saccharomyces cerevisiae 191\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 191\u003c\/p\u003e \u003cp\u003e6.2 Brief description of the methodology used 192\u003c\/p\u003e \u003cp\u003e6.2.1 Sample preparation 192\u003c\/p\u003e \u003cp\u003e6.2.2 The analysis 194\u003c\/p\u003e \u003cp\u003e6.3 Early discoveries 194\u003c\/p\u003e \u003cp\u003e6.4 Yeast stress response gives evidence of alternative pathway for glyoxylate biosynthesis in S. cerevisiae 195\u003c\/p\u003e \u003cp\u003e6.5 Biosynthesis of glyoxylate from glycine in S. cerevisiae 196\u003c\/p\u003e \u003cp\u003e6.5.1 Stable isotope labeling experiment to investigate glycine catabolism in S. cerevisiae 198\u003c\/p\u003e \u003cp\u003e6.5.2 Data leveraged for speculation 201\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Microbial Metabolomics: Rapid Sampling Techniques to Investigate Intracellular Metabolite Dynamics—An Overview 203\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 203\u003c\/p\u003e \u003cp\u003e7.2 Starting with a simple sampling device proposed by Theobald et al. (1993) 204\u003c\/p\u003e \u003cp\u003e7.3 An improved device reported by Lange et al. (2001) 205\u003c\/p\u003e \u003cp\u003e7.4 Sampling tube device by Weuster-Botz (1997) 207\u003c\/p\u003e \u003cp\u003e7.5 Fully automated device by Schaefer et al. (1999) 209\u003c\/p\u003e \u003cp\u003e7.6 The stopped-flow technique by Buziol et al. (2002) 209\u003c\/p\u003e \u003cp\u003e7.7 The BioScope: a system for continuous-pulse experiments 212\u003c\/p\u003e \u003cp\u003e7.8 Conclusions and perspectives 213\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Plant Metabolomics 215\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 215\u003c\/p\u003e \u003cp\u003e8.2 History of plant metabolomics 217\u003c\/p\u003e \u003cp\u003e8.3 Plants their metabolism and metabolomics 219\u003c\/p\u003e \u003cp\u003e8.3.1 Plant structures 219\u003c\/p\u003e \u003cp\u003e8.3.2 Plant metabolism 222\u003c\/p\u003e \u003cp\u003e8.4 Specific challenges in plant metabolomics 223\u003c\/p\u003e \u003cp\u003e8.4.1 Light dependency of plant metabolism 223\u003c\/p\u003e \u003cp\u003e8.4.2 Extraction of plant metabolites 225\u003c\/p\u003e \u003cp\u003e8.4.3 Many cell types in one tissue 225\u003c\/p\u003e \u003cp\u003e8.4.4 The dynamical range of plant metabolites 226\u003c\/p\u003e \u003cp\u003e8.4.5 Complexity of the plant metabolome 226\u003c\/p\u003e \u003cp\u003e8.4.6 Development of databases for metabolomics-derived data in plant science 228\u003c\/p\u003e \u003cp\u003e8.5 Applications of metabolomics approaches in plant research 229\u003c\/p\u003e \u003cp\u003e8.5.1 Phenotyping 229\u003c\/p\u003e \u003cp\u003e8.5.2 Functional genomics 231\u003c\/p\u003e \u003cp\u003e8.5.3 Fluxomics 232\u003c\/p\u003e \u003cp\u003e8.5.4 Metabolic trait analysis 232\u003c\/p\u003e \u003cp\u003e8.5.5 Systems biology 234\u003c\/p\u003e \u003cp\u003e8.6 Future perspectives 234\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Mass Profiling of Fungal Extract from Penicillium Species 239\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 239\u003c\/p\u003e \u003cp\u003e9.2 Methodology for screening of fungi by DiMS 242\u003c\/p\u003e \u003cp\u003e9.2.1 Cultures 243\u003c\/p\u003e \u003cp\u003e9.2.2 Extraction 243\u003c\/p\u003e \u003cp\u003e9.2.3 Analysis by direct infusion mass spectrometry 244\u003c\/p\u003e \u003cp\u003e9.3 Discussion 245\u003c\/p\u003e \u003cp\u003e9.3.1 Initial data processing 245\u003c\/p\u003e \u003cp\u003e9.3.2 Metabolite prediction 246\u003c\/p\u003e \u003cp\u003e9.3.3 Chemical diversity and similarity 248\u003c\/p\u003e \u003cp\u003e9.4 Conclusion 252\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Metabolomics in Humans and Other Mammals 253\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 253\u003c\/p\u003e \u003cp\u003e10.2 A brief history of mammalian metabolomics 257\u003c\/p\u003e \u003cp\u003e10.3 Sample preparation for mammalian metabolomics studies 260\u003c\/p\u003e \u003cp\u003e10.3.1 Working with blood 262\u003c\/p\u003e \u003cp\u003e10.3.2 Working with urine 263\u003c\/p\u003e \u003cp\u003e10.3.3 Working with cerebrospinal fluid 264\u003c\/p\u003e \u003cp\u003e10.3.4 Working with cells and tissues 267\u003c\/p\u003e \u003cp\u003e10.4 Sample analysis 268\u003c\/p\u003e \u003cp\u003e10.4.1 GC-MS analysis of urine plasma and CSF 269\u003c\/p\u003e \u003cp\u003e10.4.2 LC-MS analysis of urine blood and CFS 271\u003c\/p\u003e \u003cp\u003e10.4.3 NMR analysis of CSF urine and blood 274\u003c\/p\u003e \u003cp\u003e10.5 Applications 277\u003c\/p\u003e \u003cp\u003e10.5.1 Identification and classification of metabolic disorders 278\u003c\/p\u003e \u003cp\u003e10.6 Future outlook 283\u003c\/p\u003e \u003cp\u003eIndex 289\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Chemistry [\u003ca title=\"See our other books on Chemistry\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Chemistry%20%5BPN%5D%22\"\u003ePN\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley-Interscience","offers":[{"title":"Brand New","offer_id":52298049519896,"sku":"9780471743446","price":89.47,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780471743446.jpg?v=1781732822","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/metabolome-analysis-an-introduction-hardback-9780471743446","provider":"Freshly Printed Books","version":"1.0","type":"link"}