{"product_id":"medical-mycology-cellular-and-molecular-techniques-hardback-9780470019238","title":"Medical Mycology; Cellular and Molecular Techniques (Hardback) 9780470019238","description":"\u003cfont face=\"Georgia\"\u003e\r\n\u003cp\u003e\u003cfont size=\"6\"\u003eMedical Mycology\u003c\/font\u003e\u003cbr\u003e\r\n\u003cfont size=\"5\"\u003eCellular and Molecular Techniques\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\r\n\u003cp\u003e\u003cfont size=\"4\"\u003eKevin Kavanagh (Edited by), K Kavanagh (Author)\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e9780470019238, Wiley\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eHardback, published 27 October 2006\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e352 pages\u003cbr\u003e25.2 x 17.4 x 2.7 cm, 0.794 kg\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\r\n\u003cp align=\"justify\"\u003e\u003cem\u003e\u003cfont size=\"3\"\u003e\"Das Buch fasst kompakt und im Sinne einer detaillierten und kommentierten Arbeitsanleitung ein breites Spektrum essentieller und aktueller Methoden fur die Wissenschaft zusammen?. Ein weiterer Bonus ist das sehr umfangreiche Literaturverzeichnis nach jedem Kapitel und die hohe Aktualitat aller Beitrage, die den Stand bei Drucklegung wiedergeben. Insgesamt ist das Buch zum Einstieg in die mykologische Forschung und als methodisches Nachschlagewerk im Labor sehr gut geeignet.\"\u003cbr\u003e J Lab Med, Marz 2008\u003cbr\u003e\u003c\/font\u003e\u003c\/em\u003e\u003c\/p\u003e\r\n\r\n\u003cp align=\"justify\"\u003e\u003cstrong\u003e\u003cfont size=\"3\"\u003eIn aller Ausfuhrlichkeit stellt Ihnen dieser Band die medizinische Mykologioe vor. Fuhrende Wissenschaftler auf diesem Gebiet erlautern Grundlagen und wichtige experimentelle Verfahren.\u003c\/font\u003e\u003c\/strong\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003e\u003cp\u003ePreface xiii\u003c\/p\u003e \u003cp\u003eList of Contributors xv\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Diagnosis of Candida Infection in Tissue by Immunohistochemistry 1\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMalcolm D. Richardson, Riina Rautemaa and Jarkko Hietanen\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 1\u003c\/p\u003e \u003cp\u003e1.2 Specificity of monoclonal antibody 3H8 for C. albicans 3\u003c\/p\u003e \u003cp\u003eProtocol 1.1 Testing of specificity of monoclonal antibody 3H8 4\u003c\/p\u003e \u003cp\u003e1.3 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms 6\u003c\/p\u003e \u003cp\u003eProtocol 1.2 Evaluation of monoclonal antibody 3H8 for the detection of C. albicans morphological forms 6\u003c\/p\u003e \u003cp\u003e1.4 Application of immunohistochemistry in the diagnosis of Candida periodontal disease 7\u003c\/p\u003e \u003cp\u003eProtocol 1.3 Use of monoclonal antibody 3H8 in the detection of C. albicans in tissue 8\u003c\/p\u003e \u003cp\u003e1.5 References 11\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Transmission Electron Microscopy of Pathogenic Fungi 13\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eGuy Tronchin and Jean-Philippe Bouchara\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 13\u003c\/p\u003e \u003cp\u003e2.2 Glutaraldehyde-potassium-permanganate or glutaraldehyde-osmiumtetroxide fixation for ultrastructural analysis 16\u003c\/p\u003e \u003cp\u003eProtocol 2.1 Glutaraldehyde-osmium tetroxide (#) or glutaraldehyde-potassium permanganate (*) fixation for ultrastructural analysis 17\u003c\/p\u003e \u003cp\u003e2.3 Identification of the different compartments of the secretory pathway in yeasts 18\u003c\/p\u003e \u003cp\u003eProtocol 2.2 Identification of the different compartments of the secretory pathway in yeasts 19\u003c\/p\u003e \u003cp\u003e2.4 Cytochemical localization of acid phosphatase in yeasts 20\u003c\/p\u003e \u003cp\u003eProtocol 2.3 Cytochemical localization of acid phosphatase in yeasts 21\u003c\/p\u003e \u003cp\u003e2.5 Detection of anionic sites 23\u003c\/p\u003e \u003cp\u003eProtocol 2.4 Detection of anionic sites 23\u003c\/p\u003e \u003cp\u003e2.6 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg) 25\u003c\/p\u003e \u003cp\u003eProtocol 2.5 Detection of glycoconjugates by the periodic acid-thiocarbohydrazide-silver proteinate technique (PATAg) 26\u003c\/p\u003e \u003cp\u003e2.7 Enzyme-gold approach for the detection of polysaccharides in the cell wall 28\u003c\/p\u003e \u003cp\u003eProtocol 2.6 Enzyme-gold approach for the detection of polysaccharides in the cell wall 29\u003c\/p\u003e \u003cp\u003e2.8 Detection of glycoconjugates by the lectin-gold technique 30\u003c\/p\u003e \u003cp\u003eProtocol 2.7 Detection of glycoconjugates by the lectin-gold technique 31\u003c\/p\u003e \u003cp\u003e2.9 Immunogold detection of antigens on ultrathin sections of acrylic resin 33\u003c\/p\u003e \u003cp\u003eProtocol 2.8 Immunogold detection of antigens on ultrathin sections of acrylic resin 34\u003c\/p\u003e \u003cp\u003e2.10 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection 36\u003c\/p\u003e \u003cp\u003eProtocol 2.9 Cryofixation and freeze substitution for ultrastructural analysis and immunodetection 37\u003c\/p\u003e \u003cp\u003e2.11 Overview 38\u003c\/p\u003e \u003cp\u003e2.12 References 39\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Evaluation of Molecular Responses and Antifungal Activity of Phagocytes to Opportunistic Fungi 43\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMaria Simitsopoulou and Emmanuel Roilides\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 43\u003c\/p\u003e \u003cp\u003e3.2 Preparation of conidia and hyphae of opportunistic fungi 45\u003c\/p\u003e \u003cp\u003eProtocol 3.1 Preparation of conidia and hyphae of opportunistic fungi 45\u003c\/p\u003e \u003cp\u003eProtocol 3.2 Preparation of hyphal fragments 47\u003c\/p\u003e \u003cp\u003e3.3 Isolation of human monocytes from whole blood 48\u003c\/p\u003e \u003cp\u003eProtocol 3.3 Isolation of human MNCs from whole blood 48\u003c\/p\u003e \u003cp\u003e3.4 Analysis of human MNC function in response to fungal infection 51\u003c\/p\u003e \u003cp\u003eProtocol 3.4 XTT microassay 52\u003c\/p\u003e \u003cp\u003eProtocol 3.5 Superoxide anion assay in 96-well plate 53\u003c\/p\u003e \u003cp\u003eProtocol 3.6 Hydrogen peroxide-rhodamine assay 55\u003c\/p\u003e \u003cp\u003eProtocol 3.7 Phagocytosis assay 55\u003c\/p\u003e \u003cp\u003e3.5 Evaluation of immunomodulators in response to fungal infection 57\u003c\/p\u003e \u003cp\u003eProtocol 3.8 Analysis of gene expression by RT-PCR 58\u003c\/p\u003e \u003cp\u003eProtocol 3.9 Analysis of pathway-specific gene expression by microarray technology 63\u003c\/p\u003e \u003cp\u003eProtocol 3.10 Assessment of cytokines and chemokines by ELISA 66\u003c\/p\u003e \u003cp\u003e3.6 Overview 67\u003c\/p\u003e \u003cp\u003e3.7 References 68\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Determination of the Virulence Factors of Candida Albicans and Related Yeast Species 69\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eKhaled H. Abu-Elteen and Mawieh Hamad\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 69\u003c\/p\u003e \u003cp\u003e4.2 Measurement of Candida species adhesion in vitro 70\u003c\/p\u003e \u003cp\u003eProtocol 4.1 The visual assessment of candidal adhesion to BECs 70\u003c\/p\u003e \u003cp\u003eProtocol 4.2 The radiometric measurement of candidal adhesion 73\u003c\/p\u003e \u003cp\u003e4.3 Adhesion to inanimate surfaces 75\u003c\/p\u003e \u003cp\u003eProtocol 4.3 Assessment of candidal adhesion to denture acrylic material 75\u003c\/p\u003e \u003cp\u003eProtocol 4.4 Adherence of Candida to plastic catheter surfaces 76\u003c\/p\u003e \u003cp\u003e4.4 C. albicans strain differentiation 77\u003c\/p\u003e \u003cp\u003eProtocol 4.5 Resistogram typing 77\u003c\/p\u003e \u003cp\u003eProtocol 4.6 The slide agglutination technique 79\u003c\/p\u003e \u003cp\u003eProtocol 4.7 Serotyping of C. albicans by flow cytomerty 80\u003c\/p\u003e \u003cp\u003e4.5 Phenotypic switching in C. albicans 81\u003c\/p\u003e \u003cp\u003eProtocol 4.8 Evaluation of phenotype switching in C. albicans 82\u003c\/p\u003e \u003cp\u003e4.6 Extracellular enzymes secreted by C. albicans 83\u003c\/p\u003e \u003cp\u003eProtocol 4.9 Measurement of extracellular proteinase production by C. albicans 85\u003c\/p\u003e \u003cp\u003eProtocol 4.10 Measurement of extracellular proteinase produced by C. albicans (staib method) 86\u003c\/p\u003e \u003cp\u003eProtocol 4.11 Measurement of extracellular phospholipases of C. albicans 87\u003c\/p\u003e \u003cp\u003e4.7 Germ-tube formation in C. albicans 88\u003c\/p\u003e \u003cp\u003eProtocol 4.12 Germ-tube formation assay 88\u003c\/p\u003e \u003cp\u003e4.8 References 89\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Analysis of Drug Resistance in Pathogenic Fungi 93\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eGary P. Moran, Emmanuelle Pinjon, David C. Coleman and Derek J. Sullivan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 93\u003c\/p\u003e \u003cp\u003e5.2 Method for the determination of minimum inhibitory concentrations (MICs) of antifungal agents for yeasts 96\u003c\/p\u003e \u003cp\u003eProtocol 5.1 Method for the determination of minimum inhibitory concentrations (MICs) of antifungal agents for yeasts 97\u003c\/p\u003e \u003cp\u003e5.3 Measurement of Rhodamine 6G uptake and glucose-induced efflux by ABC transporters 102\u003c\/p\u003e \u003cp\u003eProtocol 5.2 Measurement of rhodamine 6G uptake and glucose-induced efflux 102\u003c\/p\u003e \u003cp\u003e5.4 Analysis of expression of multidrug transporters in pathogenic fungi 105\u003c\/p\u003e \u003cp\u003e5.5 Analysis of point mutations in genes encoding cytochrome P- 450 lanosterol demethylase 106\u003c\/p\u003e \u003cp\u003e5.6 Qualitative detection of alterations in membrane sterol contents 108\u003c\/p\u003e \u003cp\u003eProtocol 5.3 Qualitative detection of alterations in membrane sterol contents 109\u003c\/p\u003e \u003cp\u003e5.7 Overview 110\u003c\/p\u003e \u003cp\u003e5.8 References 110\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Animal Models for Evaluation of Antifungal Efficacy Against Filamentous Fungi 115\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eEric Dannaoui\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 115\u003c\/p\u003e \u003cp\u003e6.2 Disseminated zygomycosis in non-immunosuppressed mice 118\u003c\/p\u003e \u003cp\u003eProtocol 6.1 Disseminated zygomycosis in non-immunosuppressed mice 118\u003c\/p\u003e \u003cp\u003e6.3 Animal model of disseminated aspergillosis 125\u003c\/p\u003e \u003cp\u003eProtocol 6.2 Disseminated aspergillosis in neutropoenic mice 126\u003c\/p\u003e \u003cp\u003e6.4 Study design for evaluation of antifungal combinations therapy in animal models 130\u003c\/p\u003e \u003cp\u003eProtocol 6.3 Study design for the evaluation of combination therapy in animal models 131\u003c\/p\u003e \u003cp\u003e6.5 References 133\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Proteomic Analysis of Pathogenic Fungi 137\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAlan Murphy\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 137\u003c\/p\u003e \u003cp\u003eProtocol 7.1 2D SDS-PAGE of protein samples 139\u003c\/p\u003e \u003cp\u003e7.2 Protein digestion in preparation for mass spectrometry by MALDI-TOF 140\u003c\/p\u003e \u003cp\u003eProtocol 7.2 Peptide mass fingerprinting (PMF) by MALDI-TOF mass spectrometry 141\u003c\/p\u003e \u003cp\u003eProtocol 7.2a In-gel digestion 142\u003c\/p\u003e \u003cp\u003eProtocol 7.2b In-solution digestion 143\u003c\/p\u003e \u003cp\u003e7.3 MALDI-TOF mass spectrometry 145\u003c\/p\u003e \u003cp\u003eProtocol 7.3 Preparation of matrix for MALDI-TOF 147\u003c\/p\u003e \u003cp\u003e7.4 Peptide mass fingerprinting (PMF) 149\u003c\/p\u003e \u003cp\u003eProtocol 7.4 Post-source decay (PSD) and chemically assisted fragmentation (CAF) 150\u003c\/p\u003e \u003cp\u003e7.5 Interpreting MALDI-TOF result spectra 152\u003c\/p\u003e \u003cp\u003e7.6 Overview 156\u003c\/p\u003e \u003cp\u003e7.7 References 157\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Extraction and Detection of DNA and RNA from Yeast 159\u003cbr\u003e \u003c\/b\u003e\u003ci\u003ePatrick Geraghty and Kevin Kavanagh\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 159\u003c\/p\u003e \u003cp\u003e8.2 The extraction of yeast DNA with the aid of phenol: chloroform 161\u003c\/p\u003e \u003cp\u003eProtocol 8.1 Whole-cell DNA extraction from C. albicans using phenol: chloroform 161\u003c\/p\u003e \u003cp\u003eProtocol 8.2 Rapid extraction of DNA from C. albicans colonies for PCR 164\u003c\/p\u003e \u003cp\u003e8.3 Detection of yeast DNA using radio-labelled probes 165\u003c\/p\u003e \u003cp\u003eProtocol 8.3 DNA detection by Southern blotting 165\u003c\/p\u003e \u003cp\u003e8.4 Extraction of whole-cell RNA using two different protocols 169\u003c\/p\u003e \u003cp\u003eProtocol 8.4 The extraction of whole-cell RNA from yeast using phenol: chloroform 170\u003c\/p\u003e \u003cp\u003eProtocol 8.5 Rapid extraction of whole-yeast-cell RNA 172\u003c\/p\u003e \u003cp\u003e8.5 Detection and expression levels of specific genes by the examination of mRNA in yeast 174\u003c\/p\u003e \u003cp\u003eProtocol 8.6 Examining mRNA content as a means of investigating gene-expression profile by northern blot analysis 175\u003c\/p\u003e \u003cp\u003eProtocol 8.7 Examining mRNA content as a means of investigating gene-expression profile by RT-PCR analysis 176\u003c\/p\u003e \u003cp\u003e8.6 References 179\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Microarrays for Studying Pathogenicity in Candida Albicans 181\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAndré Nantel, Tracey Rigby, Hervé Hogues and Malcolm Whiteway\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 181\u003c\/p\u003e \u003cp\u003e9.2 DNA microarrays 182\u003c\/p\u003e \u003cp\u003e9.3 Building a second-generation 2-colour long oligonucleotide microarray for C. albicans 183\u003c\/p\u003e \u003cp\u003eProtocol 9.1 Isolation of C. albicans RNA 185\u003c\/p\u003e \u003cp\u003eProtocol 9.2 Isolation of total RNA using the hot phenol method 186\u003c\/p\u003e \u003cp\u003eProtocol 9.3 Isolation of Poly-A+ mRNA 187\u003c\/p\u003e \u003cp\u003eProtocol 9.4 Determination of the efficiency of incorporation of the probe 192\u003c\/p\u003e \u003cp\u003eProtocol 9.5 Hybridization to DNA microarrays 194\u003c\/p\u003e \u003cp\u003e9.4 Experiment design 196\u003c\/p\u003e \u003cp\u003e9.5 Microarray-based studies in C. albicans 200\u003c\/p\u003e \u003cp\u003e9.6 Conclusion 205\u003c\/p\u003e \u003cp\u003e9.7 References 205\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Molecular Techniques for Application with Aspergillus Fumigatus 211\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eNir Osherov and Jacob Romano\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 211\u003c\/p\u003e \u003cp\u003e10.2 Preparation of knockout vectors for gene disruption and deletion in A. fumigatus 212\u003c\/p\u003e \u003cp\u003eProtocol 10.1 Preparation of knockout vectors for gene disruption and deletion in A. fumigatus 213\u003c\/p\u003e \u003cp\u003e10.3 Transformation of A. fumigatus 217\u003c\/p\u003e \u003cp\u003eProtocol 10.2 Chemical transformation of A. fumigatus 218\u003c\/p\u003e \u003cp\u003e10.4 Molecular verification of correct single integration (PCR-based) 220\u003c\/p\u003e \u003cp\u003eProtocol 10.3 Molecular verification of correct integration by PCR 221\u003c\/p\u003e \u003cp\u003e10.5 General strategies for the phenotypic characterization of A. fumigatus mutant strains 223\u003c\/p\u003e \u003cp\u003eProtocol 10.4 General strategies for the phenotypic characterization of mutants 223\u003c\/p\u003e \u003cp\u003e10.6 References 229\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Promoter Analysis and Generation of Knock-out Mutants in Aspergillus Fumigatus 231\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMatthias Brock, Alexander Gehrke, Venelina Sugareva and Axel A. Brakhage\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 231\u003c\/p\u003e \u003cp\u003e11.2 Site-directed mutagenesis of promoter elements 233\u003c\/p\u003e \u003cp\u003eProtocol 11.1 Site-directed mutation of promoter elements 233\u003c\/p\u003e \u003cp\u003e11.3 lacZ as a reporter gene 236\u003c\/p\u003e \u003cp\u003eProtocol 11.2 lacZ as a reporter gene: discontinuous determination of b-galactosidase activity 237\u003c\/p\u003e \u003cp\u003eProtocol 11.3 lacZ as a reporter gene: continuous determination of b-galactosidase activity 239\u003c\/p\u003e \u003cp\u003e11.4 Transformation of A. fumigatus 241\u003c\/p\u003e \u003cp\u003eProtocol 11.4 Transformation of A. fumigatus 242\u003c\/p\u003e \u003cp\u003e11.5 Hygromycin B as a selection marker for transformation 246\u003c\/p\u003e \u003cp\u003eProtocol 11.5 Hygromycin B as a selection marker for transformation 247\u003c\/p\u003e \u003cp\u003e11.6 pyrG as a selection marker for transformation 249\u003c\/p\u003e \u003cp\u003eProtocol 11.6 pyrG as a selection marker for transformation 249\u003c\/p\u003e \u003cp\u003e11.7 URA-blaster (A. niger pyrG) as a reusable selection marker system for gene deletions\/disruptions 251\u003c\/p\u003e \u003cp\u003eProtocol 11.7 URA-blaster (A. niger pyrG) as a reusable selection marker system for gene deletions\/disruptions 253\u003c\/p\u003e \u003cp\u003e11.8 References 255\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Microarray Technology for Studying the Virulence of Aspergillus Fumigatus 257\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eDarius Armstrong-James and Thomas Rogers\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 257\u003c\/p\u003e \u003cp\u003e12.2 Isolation of RNA from A. fumigatus 259\u003c\/p\u003e \u003cp\u003eProtocol 12.1 Isolation of total RNA from A. fumigatus 260\u003c\/p\u003e \u003cp\u003e12.3 Reverse transcription of RNA and fluorescent labelling of cDNA 263\u003c\/p\u003e \u003cp\u003eProtocol 12.2 Indirect labelling of cDNA with fluorescent dyes 264\u003c\/p\u003e \u003cp\u003e12.4 Hybridization of fluorescent probes to DNA microarrays and post-hybridization washing 266\u003c\/p\u003e \u003cp\u003eProtocol 12.3 Hybridization of fluorescent probes to DNA microarrays and post-hybridization washing 267\u003c\/p\u003e \u003cp\u003e12.5 Image acquisition from hybridized microarrays 269\u003c\/p\u003e \u003cp\u003eProtocol 12.4 Image acquisition from hybridized microarrays 269\u003c\/p\u003e \u003cp\u003e12.6 Microarray image analysis 270\u003c\/p\u003e \u003cp\u003eProtocol 12.5 Microarray image analysis 271\u003c\/p\u003e \u003cp\u003e12.7 References 272\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Techniques and Strategies for Studying Virulence Factors in Cryptococcus Neoformans 275\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eNancy Lee and Guilhem Janbon\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 275\u003c\/p\u003e \u003cp\u003e13.2 Construction of C. neoformans gene-disruption cassettes 278\u003c\/p\u003e \u003cp\u003eProtocol 13.1 Construction of C. neoformans gene-disruption cassettes 278\u003c\/p\u003e \u003cp\u003e13.3 Genetic transformation of C. neoformans 283\u003c\/p\u003e \u003cp\u003eProtocol 13.2 Biolistic transformation of C. neoformans 283\u003c\/p\u003e \u003cp\u003eProtocol 13.3 Transformation via electroporation 286\u003c\/p\u003e \u003cp\u003e13.4 Extraction of genomic DNA from C. neoformans 287\u003c\/p\u003e \u003cp\u003eProtocol 13.4 DNA for use in library construction and hybridization analysis 288\u003c\/p\u003e \u003cp\u003eProtocol 13.5 DNA for use in PCR 291\u003c\/p\u003e \u003cp\u003e13.5 Screening and identification of deletion strains 292\u003c\/p\u003e \u003cp\u003eProtocol 13.6 Screening and identification of deletion strains 292\u003c\/p\u003e \u003cp\u003e13.6 Measuring capsule size in C. neoformans 297\u003c\/p\u003e \u003cp\u003eProtocol 13.7 Measuring capsule size in C. neoformans 298\u003c\/p\u003e \u003cp\u003e13.7 Purification of glucuronoxylomannan (GXM) 298\u003c\/p\u003e \u003cp\u003eProtocol 13.8 Purification of glucuronoxylomannan (GXM) 299\u003c\/p\u003e \u003cp\u003e13.8 References 301\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Genetic Manipulation of Zygomycetes 305\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAshraf S. Ibrahim and Christopher D. Skory\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction 305\u003c\/p\u003e \u003cp\u003e14.2 Genetic tools to manipulate mucorales 306\u003c\/p\u003e \u003cp\u003e14.3 Selectable markers used with mucorales fungi 307\u003c\/p\u003e \u003cp\u003e14.4 Introduction of DNA used for transformation 308\u003c\/p\u003e \u003cp\u003eProtocol 14.1 Protoplasting of R. oryzae 309\u003c\/p\u003e \u003cp\u003eProtocol 14.2 Biolistic delivery system transformation of R. oryzae 313\u003c\/p\u003e \u003cp\u003eProtocol 14.3 A. tumefaciens-mediated transformation 316\u003c\/p\u003e \u003cp\u003e14.5 Molecular analysis of transformants 319\u003c\/p\u003e \u003cp\u003e14.6 Overview 322\u003c\/p\u003e \u003cp\u003e14.7 References 323\u003c\/p\u003e \u003cp\u003eIndex 327\u003c\/p\u003e\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\u003cp\u003e\u003cfont size=\"3\"\u003eSubject Areas: Biology, life sciences [\u003ca title=\"See our other books on Biology, life sciences\" href=\"https:\/\/freshlyprintedbooks.co.uk\/search?q=%22Biology,%20life%20sciences%20%5BPS%5D%22\"\u003ePS\u003c\/a\u003e]\u003c\/font\u003e\u003c\/p\u003e\r\n\r\n\r\n\u003c\/font\u003e","brand":"Wiley","offers":[{"title":"Brand New","offer_id":52255797051672,"sku":"9780470019238","price":67.39,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0730\/2037\/5320\/files\/9780470019238.jpg?v=1781274149","url":"https:\/\/freshlyprintedbooks.co.uk\/products\/medical-mycology-cellular-and-molecular-techniques-hardback-9780470019238","provider":"Freshly Printed Books","version":"1.0","type":"link"}